Calcein-AM
Calcein-AM (CAS: 148504-34-1), with the molecular formula C46H46N2O23, is a non-fluorescent, cell-permeable derivative of calcein. Upon intracellular hydrolysis by esterases, it yields the intensely fluorescent calcein. This characteristic makes it valuable for assessing cell viability and for studying multidrug resistance protein (MRP) activity, particularly in tumour research. Calcein-AM serves as a crucial reagent in various cell-based assays.
Cell Viability Assessment
Calcein-AM is widely used to determine the viability of cells. Once inside living cells, esterases cleave the acetoxymethyl groups, releasing the fluorescent calcein, which indicates cellular integrity and metabolic activity.
Multidrug Resistance (MDR) Studies
This compound acts as a neutral substrate for multidrug resistance-associated proteins (MRPs). Its uptake and retention can be monitored to study the efflux activity of these transporters, which is relevant in tumour biology and drug development.
Fluorescence Microscopy and Flow Cytometry
The robust fluorescence of calcein makes Calcein-AM suitable for various imaging techniques. It can be employed in fluorescence microscopy and flow cytometry to visualise and quantify live cell populations.
| Molecular weight | 994.86 |
|---|---|
| Empirical formula | C46H46N2O23 |
| Assay | ≥90% (HPLC) |
| Solubility | DMSO: soluble10 mg/mL, clear, colorless to slightly yellow |
| Concentration | ≥4 mM in DMSO |
| Storage temperature | −20°C |
| Application | Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+ |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Calcein-AM used for?
+
Calcein-AM is used as a cell-permeable substrate to assess cell viability and to study the activity of multidrug resistance proteins (MRPs), particularly in tumour research. It becomes fluorescent upon intracellular hydrolysis by esterases.
What is the CAS number and molecular formula for Calcein-AM?
+
The CAS number for Calcein-AM is 148504-34-1, and its molecular formula is C46H46N2O23.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies Calcein-AM with a purity of ≥90% (HPLC). TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should Calcein-AM be handled and stored?
+
Handle Calcein-AM with appropriate personal protective equipment, including eyeshields, gloves, and a P1 (EN143) or N95 respirator filter. Store the product at -20°C.
How is Calcein-AM packaged and shipped?
+
Calcein-AM is available in various pack sizes and is shipped globally by Tech Serve Solutions, adhering to all necessary export regulations for fine chemicals.
How can I request a quote or sample for Calcein-AM?
+
To request a quote or a sample of Calcein-AM, please contact our sales team through the website or via email, providing your specific requirements.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis