Calcein Blue
Calcein Blue (CAS 54375-47-2), with the molecular formula C15H15NO7, is a metallofluorochromic indicator. It is primarily utilised in analytical chemistry for the EDTA titration of calcium at elevated pH levels. Additionally, it serves as an indicator for copper, nickel, and cobalt titrations under acidic conditions. Its fluorescent properties, with an excitation wavelength of 322 nm and emission wavelength of 445 nm in methanol, make it a valuable tool in specific quantitative analyses.
- IUPAC
- 4-Methylumbelliferone-8-methyliminodiacetic acid
EDTA Titration of Calcium
Calcein Blue functions as a metallofluorochromic indicator for the precise determination of calcium ions using EDTA titration. This method is particularly effective at high pH values.
Transition Metal Ion Titration
The compound is also employed as an indicator in the EDTA titration of transition metals such as copper, nickel, and cobalt. These titrations are typically performed under low pH conditions.
Fluorescent Indicator
Its distinct fluorescent characteristics, with excitation and emission maxima in methanol, allow for its use as a sensitive probe in various analytical procedures where fluorescence detection is advantageous.
| Molecular weight | 321.28 |
|---|---|
| Empirical formula | C15H15NO7 |
| Application | A metallofluorochromic indicator for EDTA titration of calcium at high pH and copper, nickel and cobalt at low pH. |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 322 nm; λem 445 nm in methanol |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Calcein Blue used for?
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Calcein Blue (CAS 54375-47-2) is used as a metallofluorochromic indicator for the EDTA titration of calcium at high pH and copper, nickel, and cobalt at low pH.
What are the CAS number and formula for Calcein Blue?
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The CAS number for Calcein Blue is 54375-47-2, and its molecular formula is C15H15NO7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Calcein Blue as a research-grade chemical. We do not represent this product as meeting USP, BP, or EP pharmacopoeial standards.
How should Calcein Blue be handled safely?
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Calcein Blue is classified with GHS07 signal word 'Warning', presenting hazards H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation). Handle with appropriate personal protective equipment, including a dust mask (type N95), eyeshields, and gloves. Ensure adequate ventilation and avoid dust formation. Follow P261, P305 + P351 + P338 precautions.
How is Calcein Blue packed and shipped?
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Calcein Blue is carefully packed to ensure product integrity during transit and is exported globally by Tech Serve Solutions, adhering to all relevant shipping regulations.
How can I request a sample or quote for Calcein Blue?
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To request a sample or a quote for Calcein Blue, please contact our sales team through the website or by phone, providing your specific requirements.
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