Calcein
Calcein (CAS: 154071-48-4; Formula: C30H26N2O13) is a fluorescent compound supplied by TSS as a powder. It exhibits specific fluorescence properties with an excitation maximum of 470 nm and an emission maximum of 509 nm at pH 7.4. Calcein is recognised for its utility as a calcium probe, playing a key role in cellular research for monitoring intracellular calcium levels and in various analytical applications. This compound is available for research and analytical purposes.
- IUPAC
- Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein,Fluorescein-bis(methyliminodiacetic acid),Fluorexon
- Synonyms
- Bis[N,N−bis(carboxymethyl)aminomethyl]fluoresceinFluorescein-bis(methyliminodiacetic acid)Fluorexon
Calcium Imaging and Sensing
Calcein serves as a vital fluorescent indicator for intracellular calcium concentration. Its ability to bind calcium ions and exhibit a change in fluorescence intensity makes it invaluable for real-time monitoring of calcium dynamics in living cells.
Cell Viability and Membrane Integrity Assays
Due to its fluorescence properties and ability to permeate intact cell membranes, Calcein is utilized in assays to assess cell viability and to detect compromised membrane integrity, often employed in toxicology and drug screening.
Analytical Chemistry Reagent
As a chemical reagent, Calcein finds application in analytical procedures, particularly those involving spectrophotometry or fluorometry, owing to its distinct spectral characteristics. It is also used in chromatography.
Cell Biology Research
This compound is a key tool in cell biology for studying cellular processes dependent on calcium signalling. Its applications extend to neuroscience and other fields investigating cellular communication and function.
| Molecular weight | 622.53 |
|---|---|
| Empirical formula | C30H26N2O13 |
| Form | powder |
| Solubility | NaOH: soluble50 mg/mL |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Description | mixed isomers |
|---|---|
| Fluorescence | λex 470 nm; λem 509 nm (pH 7.4) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Calcein used for?
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Calcein is primarily used as a fluorescent probe for detecting intracellular calcium levels, in cell viability assays, and as a general analytical reagent in chemistry and cell biology research, including cell signalling studies.
What is the CAS number and chemical formula for Calcein?
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The CAS number for Calcein is 154071-48-4, and its empirical formula is C30H26N2O13.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Calcein as a research-grade chemical. The specific assay and purity details are available on the product's Certificate of Analysis. We do not represent this product as USP, BP, or EP grade.
What are the safety considerations when handling Calcein?
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When handling Calcein, it is essential to wear appropriate personal protective equipment, including eyeshields, gloves, and a P1 (EN143) or N95 (US) respirator filter. Calcein has a WGK Germany rating of 3, indicating it is hazardous to water.
How is Calcein packed, shipped, and exported by TSS?
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Tech Serve Solutions packs and ships Calcein according to international regulations for chemical substances. We are experienced global exporters, ensuring compliant handling and delivery.
How can I request a quote or sample of Calcein?
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To request a quote or a sample of Calcein, please contact our sales team via the website or by phone, providing your specific requirements.
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