5-Carboxyfluorescein N-succinimidyl ester
5-Carboxyfluorescein N-succinimidyl ester (CAS: 92557-80-7), with the molecular formula C25H15NO9 and a molecular weight of 473.39 g/mol, is a fluorescent labelling agent. It is derived from fluorescein and is suitable for fluorescence applications. This compound is primarily used for covalently labelling molecules with a fluorescent tag, enabling detection and analysis in various biological and chemical assays.
Fluorescent Labelling
This succinimidyl ester is designed to react with primary amines, making it ideal for labelling proteins, antibodies, peptides, and other biomolecules. The resulting conjugates exhibit fluorescence, allowing for sensitive detection in techniques like microscopy and flow cytometry.
Biochemical Assays
It serves as a valuable reagent in biochemical research for developing fluorescent probes. The ester group facilitates conjugation, and the fluorescein core provides a well-characterised fluorescent signal, useful for various analytical procedures.
Detection and Imaging
The inherent fluorescence properties of the fluorescein moiety make this compound useful for applications requiring sensitive detection and imaging. It can be employed in assays where tracking or quantifying labelled entities is necessary.
| Molecular weight | 473.39 |
|---|---|
| Empirical formula | C25H15NO9 |
| Assay | ≥90% (HPLC) |
| Storage temperature | −20°C |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Carboxyfluorescein N-succinimidyl ester used for?
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5-Carboxyfluorescein N-succinimidyl ester is used for fluorescent labelling of biomolecules such as proteins and peptides. It is suitable for applications requiring sensitive detection and imaging in biochemical assays and research.
What is the CAS number and formula for 5-Carboxyfluorescein N-succinimidyl ester?
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The CAS number is 92557-80-7 and the empirical formula is C25H15NO9.
What grade and purity does Tech Serve Solutions supply for 5-Carboxyfluorescein N-succinimidyl ester?
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Tech Serve Solutions supplies 5-Carboxyfluorescein N-succinimidyl ester with an assay of ≥90% (HPLC). It is supplied as a BioReagent and is suitable for fluorescence. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling requirements for 5-Carboxyfluorescein N-succinimidyl ester?
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Handling this compound requires personal protective equipment including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The water hazard class (WGK) in Germany is 3.
How is 5-Carboxyfluorescein N-succinimidyl ester packed, shipped, and exported?
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This chemical is stored at -20°C. Tech Serve Solutions, as a specialist global supplier and exporter, ensures appropriate packaging and documentation for the safe and compliant shipment and export of this product.
How can I request a sample or quote for 5-Carboxyfluorescein N-succinimidyl ester?
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To request a sample or a quote, please contact our sales team through the website or by phone. Please provide the product name and CAS number (92557-80-7) when making your inquiry.
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