5-Carboxy-tetramethylrhodamine N-succinimidyl ester
5-Carboxy-tetramethylrhodamine N-succinimidyl ester (CAS: 150810-68-7, Formula: C29H25N3O7) is a fluorescent labelling reagent with a molecular weight of 527.52 g/mol. This compound is used for amine coupling, enabling the derivatization of molecules with the 5-carboxy-tetramethylrhodamine (5-TAMRA) fluorophore. It is suitable for applications requiring fluorescence detection, such as in molecular probes, enzyme substrates, and FRET studies.
- IUPAC
- \"4-Carboxytetramethylrhodamine N-succinimidyl ester\",5-TAMRA
Amine Coupling Reagent
5-Carboxy-tetramethylrhodamine N-succinimidyl ester serves as an activated ester for the covalent labelling of primary amines. This reaction allows for the efficient incorporation of the 5-TAMRA fluorophore onto target molecules.
Fluorescent Probes and Labels
The 5-TAMRA moiety introduced via this reagent can be used to create fluorescently labelled biomolecules or probes for various detection assays. Its spectral properties make it suitable for microscopy and flow cytometry applications.
Enzyme Substrates
This compound can be used in the synthesis of enzyme substrates where the release of the 5-TAMRA fluorophore upon enzymatic cleavage results in a measurable fluorescent signal.
Fluorescence Resonance Energy Transfer (FRET)
5-TAMRA is often employed as a FRET donor or acceptor in biological studies. Derivatization with this ester enables the creation of FRET pairs for investigating molecular interactions and conformations.
| Molecular weight | 527.52 |
|---|---|
| Empirical formula | C29H25N3O7 |
| Solubility | DMF: solubleacetonitrile: soluble |
| Storage temperature | −20°C |
| Application | 5-Carboxy-tetramethylrhodamine N-succinimidyl ester (5-TAMRA) is used as an amine coupling reagent to form 5-carboxy-tetramethylrhodamine (5-TAMRA) derivatized compounds. 5-TAMRA derivatized compounds may be used in applications as enzyme substrates, molecular probes, molecular beacons and as fluorescence resonance energy transfer (FRET) partners. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Carboxy-tetramethylrhodamine N-succinimidyl ester used for?
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It is primarily used as an amine coupling reagent to create 5-TAMRA derivatized compounds, which find applications as enzyme substrates, molecular probes, molecular beacons, and FRET partners.
What are the CAS number and chemical formula for 5-Carboxy-tetramethylrhodamine N-succinimidyl ester?
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The CAS number is 150810-68-7 and the empirical formula (Hill notation) is C29H25N3O7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product as a BioReagent grade. TSS does not represent this product as USP, BP, or EP.
How should 5-Carboxy-tetramethylrhodamine N-succinimidyl ester be handled?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, as it is classified under WGK Germany 3.
How is this chemical shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, handling the complexities of international shipping and customs regulations for products like this.
How can I request a quote or sample?
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Please contact our sales team directly to inquire about pricing, bulk quantities, or to request a sample of 5-Carboxy-tetramethylrhodamine N-succinimidyl ester.
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