5-Carboxy-X-rhodamine N-succinimidyl ester

CAS 209734-74-7C37H33N3O7Biochemicals & Reagents

5-Carboxy-X-rhodamine N-succinimidyl ester (CAS: 209734-74-7), with the molecular formula C37H33N3O7 and a molecular weight of 631.67 g/mol, is a vital fluorescent labelling reagent. It is primarily used for covalently attaching the fluorescent dye 5-carboxy-X-rhodamine (ROX) to biomolecules such as proteins and nucleic acids. This enables applications in FRET, fluorescence quenching studies, and direct-load DNA sequencing.

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5-Carboxy-X-rhodamine N-succinimidyl ester (C37H33N3O7) — chemical structure, CAS 209734-74-7; Life Science, fine chemical supplied by Tech Serve Solutions

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01 /Applications

Amine Coupling Reagent for Rhodamine Derivatisation

5-Carboxy-X-rhodamine N-succinimidyl ester is employed to covalently link the ROX fluorophore to amine groups present in proteins, peptides, and other biomolecules. This process facilitates the creation of fluorescently labelled molecules for various biological assays and imaging techniques.

Fluorescence Resonance Energy Transfer (FRET) Studies

The resulting ROX-labelled compounds derived from this reagent are suitable for FRET applications. FRET is a powerful technique used to study molecular interactions, conformational changes, and binding events in biological systems.

Fluorescence Quenching Applications

It serves as a precursor for creating molecules used in fluorescence quenching assays. These assays can monitor molecular binding, enzymatic activity, or environmental changes by detecting alterations in fluorescence intensity.

DNA Sequencing Reagents

This compound is utilised in the preparation of charge-modified dye-labelled ddNTPs for direct-load DNA sequencing. This methodology streamlines the sequencing process by eliminating the need for post-electrophoresis labelling.

02 /Properties

Molecular weight	631.67
Empirical formula	C37H33N3O7
Solubility	DMF: solubleacetonitrile: soluble
Storage temperature	−20°C
Application	5-Carboxy-X-rhodamine N-succinimidyl ester (ROX-SE) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications.Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to \"direct-load\" DNA sequencing.1,2
Suitability	suitable for fluorescence

03 /Safety & handling

GHS hazard pictogram: Harmful / irritant (GHS07) — Harmful / irritant

Warning

Hazard statements

H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust, fume, gas or vapours
P305IF IN EYES

Protective equipment	dust mask type N95 (US), Eyeshields, Gloves
Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xi
Risk statements (R)	36/37/38
Safety statements (S)	26-36

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

209734-74-7

05 /Additional specifications

Product Line	BioReagent
Fluorescence	λex 575 nm; λem 600 nm in 0.1 M phosphate pH 7.0

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is 5-Carboxy-X-rhodamine N-succinimidyl ester used for?

This compound is used as an amine coupling reagent to create ROX-derivatised molecules, including proteins, nucleic acids, and drugs, for FRET and fluorescence quenching applications. It is also used for labelling ddNTPs in direct-load DNA sequencing.

What are the CAS number and molecular formula?

The CAS number is 209734-74-7 and the molecular formula is C37H33N3O7.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product for research use. The specific assay or purity is available on the Certificate of Analysis. TSS does not represent this product as USP, EP, BP or pharmacopoeia grade.

What are the safety and handling considerations?

This compound is a Warning substance, causing skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Use with adequate ventilation and wear appropriate personal protective equipment including eyeshields, gloves, and a type N95 dust mask.

How is this chemical supplied and exported?

We expertly package and export fine chemicals globally, adhering to all relevant shipping and handling regulations to ensure safe and timely delivery to your laboratory.

How can I request a sample or a quote?

To request a sample or a quotation, please contact our sales team through the website or call us directly. We are happy to assist with your research needs.