5-Carboxy-fluorescein diacetate N-succinimidyl ester
5-Carboxy-fluorescein diacetate N-succinimidyl ester (CAS: 150206-05-6; Formula: C29H19NO11; MW: 557.46) is a fluorescent labeling reagent. It functions as a succinimidyl ester, enabling conjugation to amine groups. This compound is valuable for introducing a fluorescein moiety into biomolecules for fluorescent detection and imaging in various biological and biochemical research applications.
- IUPAC
- 5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester
- Synonyms
- 5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

Fluorescent Labeling of Amines
This reagent covalently labels primary amine groups on proteins, peptides, and other biomolecules. The resulting fluorescent conjugates can be detected and quantified using fluorescence spectroscopy or microscopy.
Biomolecule Detection and Imaging
The fluorescein label allows for sensitive detection of labeled biomolecules in diverse biological systems. Applications include immunofluorescence, flow cytometry, and live-cell imaging.
Biochemical Assays
Used in developing fluorescent assays for studying enzyme activity, protein-protein interactions, and other biochemical processes where amine labeling is required.
| Molecular weight | 557.46 |
|---|---|
| Empirical formula | C29H19NO11 |
| Assay | ≥95.0% (HPLC) |
| Grade | for fluorescence |
| Solubility | DMF: solubleDMSO: soluble |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 492 nm; λem 517 nm |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Carboxy-fluorescein diacetate N-succinimidyl ester used for?
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5-Carboxy-fluorescein diacetate N-succinimidyl ester is primarily used for the fluorescent labeling of amine groups on biomolecules, enabling their detection and imaging in biological research.
What is the CAS number and formula for 5-Carboxy-fluorescein diacetate N-succinimidyl ester?
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The CAS number is 150206-05-6 and the molecular formula is C29H19NO11.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥95.0% (HPLC), suitable for fluorescence applications. We do not represent this product as USP, EP, BP or pharmacopoeia grade.
What are the safety and handling recommendations?
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Handling requires personal protective equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The substance has a WGK Germany of 3, indicating it is hazardous to water.
How is this chemical packed, shipped, and exported?
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This chemical is typically stored at -20°C. As a global supplier, Tech Serve Solutions adheres to all relevant international shipping regulations for chemical export.
How can I request a sample or quote for 5-Carboxy-fluorescein diacetate N-succinimidyl ester?
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Please contact our sales team through the website to request a sample or a quotation for this product.
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