5-Carboxyfluorescein
5-Carboxyfluorescein (CAS: 76823-03-5; Formula: C21H12O7; MW: 376.32) is a fluorescent dye and a derivative of fluorescein, widely utilized as a fluorescent label and probe. Its distinct fluorescent properties, with an excitation maximum at 492 nm and emission maximum at 517 nm, make it invaluable in various detection and labeling applications within biological and chemical research. TSS supplies this compound for research purposes.
- IUPAC
- 5-FAM
Fluorescent Labeling
5-Carboxyfluorescein serves as a fluorescent label for biomolecules, enabling their detection and tracking in various biological assays. Its carboxyl group allows for straightforward conjugation to proteins, antibodies, or nucleic acids.
Fluorescent Probes
It functions as a fluorescent probe in microscopy and imaging techniques. The compound's spectral characteristics are useful for visualizing cellular structures or monitoring biological processes where fluorescence detection is required.
Biochemical Detection
This compound is employed in biochemical assays for detection purposes. Its fluorescence emission under specific excitation wavelengths facilitates the quantitative analysis of biological samples.
Nucleic Acid Staining
As a derivative of fluorescein, 5-Carboxyfluorescein can be applied in staining applications for nucleic acids. It is particularly useful in microscopy for visualizing DNA or RNA within cellular contexts.
| Molecular weight | 376.32 |
|---|---|
| Empirical formula | C21H12O7 |
| Assay | 99% (HPLC) |
| Solubility | DMF: solubleDMSO: solubleH2O: soluble |
| Storage temperature | 2-8°C |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Carboxyfluorescein used for?
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5-Carboxyfluorescein is primarily used as a fluorescent label and probe in various biochemical and biological detection applications, including microscopy, imaging, and nucleic acid staining.
What are the CAS number and formula for 5-Carboxyfluorescein?
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The CAS number for 5-Carboxyfluorescein is 76823-03-5, and its empirical formula is C21H12O7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5-Carboxyfluorescein with an assay of 99% (HPLC). This product is supplied for research purposes and is not represented as USP, BP, EP, or pharmacopoeia grade.
How should 5-Carboxyfluorescein be handled and stored?
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Handle 5-Carboxyfluorescein with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Store the compound at 2-8°C.
How is 5-Carboxyfluorescein packed, shipped, and exported?
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5-Carboxyfluorescein is carefully packed to ensure stability during transport and is available for global export by Tech Serve Solutions.
How can I request a sample or quote for 5-Carboxyfluorescein?
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To request a sample or a quote for 5-Carboxyfluorescein, please contact the Tech Serve Solutions sales team through our website or direct contact channels.
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