5-Carboxytetramethylrhodamine
5-Carboxytetramethylrhodamine (CAS: 91809-66-4), with the empirical formula C25H22N2O5 and a molecular weight of 430.45 g/mol, is a fluorescent rhodamine derivative. It is widely employed as a fluorescent label and probe in various biological and cell biology applications. Its characteristic fluorescence properties, with excitation and emission maxima around 543 nm and 570 nm respectively in methanol, make it valuable for visualisation techniques in research.
- IUPAC
- 5-TAMRA
- Synonyms
- 5-TAMRA

Fluorescent Labeling
Used to covalently attach to biomolecules, enabling their detection and tracking in biological systems. Its rhodamine core provides bright fluorescence.
Cell Biology Research
Serves as a fluorescent probe to study cellular structures, processes, and dynamics. Its spectral properties are suitable for microscopy and flow cytometry.
Biochemical Assays
Incorporated into assays for detecting and quantifying biological analytes. The fluorescence signal generated can be correlated with the presence or concentration of the target.
Histology and Hematology
Applied as a stain or label in histological and hematological sample preparation for microscopic examination. It aids in differentiating cellular components.
| Molecular weight | 430.45 |
|---|---|
| Empirical formula | C25H22N2O5 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex ~543 nm; λem ~570 nm in methanol |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Carboxytetramethylrhodamine used for?
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5-Carboxytetramethylrhodamine is primarily used as a fluorescent label and probe in cell biology research, biochemical assays, and histology/hematology.
What are the CAS number and empirical formula for 5-Carboxytetramethylrhodamine?
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The CAS number is 91809-66-4, and the empirical formula is C25H22N2O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical as a research grade reagent. We do not represent it as USP, BP, or EP grade. Please refer to the Certificate of Analysis for specific purity information.
How should 5-Carboxytetramethylrhodamine be handled safely?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The product carries a WGK Germany rating of 3.
Can Tech Serve Solutions export 5-Carboxytetramethylrhodamine?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals.
How can I request a sample or quote for 5-Carboxytetramethylrhodamine?
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Please contact our sales team via the website or telephone to request a sample or a formal quotation.
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