5-Cholesten-3β-ol-7-one
5-Cholesten-3β-ol-7-one (CAS: 566-28-9), with the molecular formula C27H44O2 and a molecular weight of 400.64 g/mol, is a vital cholesterol derivative. It serves primarily as an analytical standard. This compound is essential for accurate quantification of cholesterol derivatives in various biological and chemical samples using sophisticated analytical techniques.
- IUPAC
- 3β-Hydroxy-5-cholesten-7-one,7-Ketocholesterol
- Synonyms
- 7-Ketocholesterol3β-Hydroxy-5-cholesten-7-one

GC-MS Standard
Utilised as a standard for the determination of cholesterol derivatives using Gas Chromatography-Mass Spectrometry (GC-MS). This application ensures precise and reliable identification and quantification in complex mixtures.
HPLC Standard
Employed as a reference standard in High-Performance Liquid Chromatography (HPLC) assays for cholesterol derivatives. Its use facilitates accurate chromatographic separation and quantification.
Biochemical Research
Serves as a key biochemical reagent in lipid research. Its specific structure allows for studies into steroid metabolism and related biological pathways.
| Molecular weight | 400.64 |
|---|---|
| Empirical formula | C27H44O2 |
| Assay | ≥90% |
| Application | 5-Cholesten-3β-ol-7-one was used as a standard in the determination of cholesterol derivatives by GC-MS1,2 and HPLC.3 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Cholesten-3β-ol-7-one used for?
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5-Cholesten-3β-ol-7-one is used as an analytical standard for the determination of cholesterol derivatives by GC-MS and HPLC. It is also a useful biochemical reagent in lipid research.
What are the CAS number and formula for 5-Cholesten-3β-ol-7-one?
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The CAS number is 566-28-9 and the molecular formula is C27H44O2.
What grade and purity does Tech Serve Solutions supply for 5-Cholesten-3β-ol-7-one?
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Tech Serve Solutions supplies 5-Cholesten-3β-ol-7-one with an assay of ≥90%. We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should 5-Cholesten-3β-ol-7-one be handled?
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Appropriate personal protective equipment includes eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Users should consult the safety data sheet for comprehensive handling information. This compound is classified with WGK Germany 3.
Does Tech Serve Solutions export 5-Cholesten-3β-ol-7-one?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including 5-Cholesten-3β-ol-7-one.
How can I request a quote or sample for 5-Cholesten-3β-ol-7-one?
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Please contact our sales department via phone or email to request a quote or a sample of 5-Cholesten-3β-ol-7-one.
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