5-Bromo-4-chloro-3-indolyl butyrate
5-Bromo-4-chloro-3-indolyl butyrate (CAS 129541-43-1) is a chromogenic substrate with the molecular formula C12H11BrClNO2 and a molecular weight of 316.58 g/mol. This compound is primarily utilised in biochemical and enzymatic assays for the detection of specific enzyme activities. Its ability to produce a coloured precipitate upon enzymatic cleavage makes it invaluable for various diagnostic and research applications requiring visual confirmation of enzyme presence or activity.

Chromogenic Enzyme Substrate
5-Bromo-4-chloro-3-indolyl butyrate serves as a chromogenic substrate for detecting esterase and lipase activity. Upon enzymatic hydrolysis, it releases a coloured indolyl derivative, allowing for visual identification and quantification of enzyme presence in biological samples.
Biochemical Research Reagent
This compound is employed in various biochemical research settings to study enzyme kinetics and mechanisms. Its use facilitates the development of assays for identifying enzyme inhibitors and understanding enzymatic pathways.
Diagnostic Assay Development
The chromogenic properties of 5-Bromo-4-chloro-3-indolyl butyrate are leveraged in the development of diagnostic kits and assays. It aids in the detection of specific enzyme markers relevant to various physiological and pathological conditions.
| Molecular weight | 316.58 |
|---|---|
| Empirical formula | C12H11BrClNO2 |
| Assay | ≥99% |
| Storage temperature | −20°C |


Hazard statements
- H302Harmful if swallowed
- H318Causes serious eye damage
Precautionary statements
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-41 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl butyrate used for?
+
5-Bromo-4-chloro-3-indolyl butyrate is used as a chromogenic substrate for detecting esterase and lipase activity in biochemical research and diagnostic assay development.
What is the CAS number and chemical formula for 5-Bromo-4-chloro-3-indolyl butyrate?
+
The CAS number is 129541-43-1, and the chemical formula is C12H11BrClNO2.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies 5-Bromo-4-chloro-3-indolyl butyrate with a purity of ≥99%. We do not represent this product as USP, BP, or EP grade.
How should 5-Bromo-4-chloro-3-indolyl butyrate be handled safely?
+
Handle with care, wearing appropriate personal protective equipment including a dust mask (type N95 US), eyeshields, face shield, and gloves. This compound is harmful if swallowed and can cause serious eye damage. Ensure adequate ventilation and avoid dust formation.
How is this product shipped and exported?
+
Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, capable of arranging appropriate shipping and export documentation for 5-Bromo-4-chloro-3-indolyl butyrate worldwide.
How can I request a quote or sample?
+
To request a quote or sample of 5-Bromo-4-chloro-3-indolyl butyrate, please contact our sales team through the website or by phone.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents

(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more

3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis

3-Methyl-1,5-pentanediol
Chemical Synthesis

3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis