5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt

CAS 102212-99-7C9H14BrN2O14P3Biochemicals & Reagents

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt (CAS: 102212-99-7) is a modified nucleoside triphosphate with the molecular formula C9H14BrN2O14P3. This biochemical reagent serves as a crucial substrate in enzymatic reactions for DNA synthesis and labeling. It is also recognised for its mutagenic properties, making it a valuable tool in molecular biology research for various applications, including detection via immunological or derivatized methods.

IUPAC: 5-BrdUTP

Email us

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt (C9H14BrN2O14P3) — chemical structure, CAS 102212-99-7; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsNucleosidesNucleotidesOligonucleotidesNucleotide Analogs

01 /Applications

Terminal Deoxynucleotidyl Transferase (TdT) Substrate

Used as a labeling substrate for TdT in enzymatic reactions. This allows for the specific addition of the brominated deoxyuridine moiety to DNA termini.

DNA Synthesis

Incorporated into DNA during synthesis, serving as a building block. This can be utilised for research purposes requiring modified DNA strands.

Mutagenic Agent

Functions as an effective mutagenic agent in laboratory settings. Its incorporation can lead to changes in DNA sequences, facilitating mutation studies.

Detection and Labeling

The incorporated 5-BrdUTP can be detected using immunological techniques. Alternatively, it can be derivatized with biotin or other moieties for detection via fluorogenic or chromogenic methods.

02 /Properties

Molecular weight	547.04
Empirical formula	C9H14BrN2O14P3
Assay	≥90%
Storage temperature	−20°C
Application	5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.

03 /Safety & handling

GHS hazard pictogram: Toxic (GHS06) — Toxic

Danger

Hazard statements

H301Toxic if swallowed
H311Toxic in contact with skin
H315Causes skin irritation
H319Causes serious eye irritation
H331Toxic if inhaled
H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust, fume, gas or vapours
P280Wear protective gloves, clothing and eye/face protection
P301IF SWALLOWED
P305IF IN EYES
P311Call a POISON CENTER or doctor

Protective equipment	Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
Water hazard class (WGK, DE)	3
Hazard codes (EU)	T
Risk statements (R)	23/24/25-36/37/38
Safety statements (S)	22-26-36-45

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

102212-99-7

MDL number

MFCD00057405

PubChem substance

24891527

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt used for?

This compound is primarily used as a labeling substrate for terminal deoxynucleotidyl transferase (TdT) in DNA synthesis, as an effective mutagenic agent, and for DNA labeling which can be detected immunologically or via other derivatization methods.

What are the CAS number and formula for 5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt?

The CAS number is 102212-99-7 and the empirical formula is C9H14BrN2O14P3.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies 5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt with an assay of ≥90%. We do not represent this product as USP, BP, EP, or other pharmacopoeial grades.

What are the safety and handling precautions for this chemical?

This compound is classified as Danger. It is toxic if swallowed, in contact with skin, and if inhaled, and causes skin and serious eye irritation. Appropriate personal protective equipment, including eyeshields, face shields, gloves, and P2 respirator cartridges, must be used. Handle only in a well-ventilated area or under a fume hood.

How is 5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt supplied and shipped?

This chemical is stored at -20°C and can be packed, exported, and shipped globally by Tech Serve Solutions according to your specific requirements and all relevant regulations.

How can I request a sample or quote?

Please contact our sales team via the website or direct email to request a sample or obtain a quotation for 5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt.