5-Bromo-4-chloro-3-indolyl acetate
5-Bromo-4-chloro-3-indolyl acetate (CAS 3252-36-6) is a biochemical reagent with the molecular formula C10H7BrClNO2 and a molecular weight of 288.53. It is primarily used as an enzyme substrate, particularly for detecting esterase activity. This compound is a valuable tool in biochemical research and diagnostics, enabling the visualization of enzymatic reactions through colourimetric methods.

Enzyme Substrate for Esterase Detection
5-Bromo-4-chloro-3-indolyl acetate serves as a chromogenic substrate for various esterases. Upon enzymatic cleavage, it liberates a blue precipitate, facilitating the identification and localisation of esterase activity in biological samples.
Biochemical Research Tool
This compound is employed in molecular biology and biochemistry laboratories for research purposes, aiding in the study of enzyme kinetics and specificity.
Diagnostic Assays
It can be incorporated into diagnostic kits for detecting specific enzyme activities relevant to various biological or pathological conditions.
| Molecular weight | 288.53 |
|---|---|
| Empirical formula | C10H7BrClNO2 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl acetate used for?
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5-Bromo-4-chloro-3-indolyl acetate is primarily used as a chromogenic substrate for the detection of esterase activity in biochemical research and diagnostic applications. Upon enzymatic hydrolysis, it yields a coloured product.
What are the CAS number and formula for 5-Bromo-4-chloro-3-indolyl acetate?
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The CAS number is 3252-36-6, and the empirical formula is C10H7BrClNO2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical for research and laboratory use. The exact assay and purity are detailed on the product's Certificate of Analysis. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling recommendations for 5-Bromo-4-chloro-3-indolyl acetate?
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This compound requires careful handling. Recommended personal protective equipment includes eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne exposure is possible. It is classified under WGK-Germany 3, indicating it is hazardous to water.
How is 5-Bromo-4-chloro-3-indolyl acetate packed and shipped?
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Products are carefully packed according to international shipping regulations for chemicals. Specific packaging details can be provided upon request for export.
How can I request a sample or a quote?
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To request a sample or a quote, please contact our sales department via the website or by phone, providing the product name and CAS number.
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