5-Bromo-4-chloro-3-indolyl phosphate disodium salt
5-Bromo-4-chloro-3-indolyl phosphate disodium salt (CAS: 102185-33-1) is a water-soluble chromogenic substrate for the colorimetric detection of alkaline phosphatase (AP) activity. This compound, with a molecular weight of 370.43 g/mol, is integral to various biochemical assays. It is widely employed in molecular biology techniques for sensitive and visible detection of AP-labeled probes, enabling precise localisation and quantification in numerous research applications.
- IUPAC
- BCIP®,X-phosphate disodium salt
Colorimetric Detection of Alkaline Phosphatase
This substrate is specifically designed for the colorimetric detection of alkaline phosphatase (AP) enzyme activity. Upon enzymatic cleavage, it generates an insoluble, distinct purple precipitate, facilitating visual identification of AP-labeled molecules.
Northern, Southern, and Western Blotting
It serves as a critical detection reagent in blotting techniques such as Northern, Southern, and Western blots, where AP-conjugated antibodies or nucleic acid probes are used. The enzymatic reaction leads to a localised colour formation at the target site.
In Situ Hybridization
5-Bromo-4-chloro-3-indolyl phosphate disodium salt is valuable for in situ hybridization (ISH) assays, enabling the detection of specific nucleic acid sequences within cellular or tissue contexts by visualising AP activity.
Immunohistochemistry (IHC)
In immunohistochemistry, this substrate aids in the visualisation of AP-conjugated antibodies, allowing for the precise localisation of antigens within tissue samples through the formation of a coloured product.
| Molecular weight | 370.43 |
|---|---|
| Linear formula | C8H4BrClNO4P · 2Na |
| Assay | ≥98% (HPLC) |
| Solubility | H2O: soluble20 mg/mLDMF: insoluble |
| Storage temperature | −20°C |
| Application | For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl phosphate disodium salt used for?
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This compound is primarily used as a chromogenic substrate for the colorimetric detection of alkaline phosphatase (AP) activity. It is essential in molecular biology techniques like Northern, Southern, and Western blotting, in situ hybridization, and immunohistochemistry for visualising AP-labeled molecules.
What is the CAS number and linear formula for this product?
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The CAS number is 102185-33-1, and the linear formula is C8H4BrClNO4P · 2Na.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5-Bromo-4-chloro-3-indolyl phosphate disodium salt with an assay of ≥98% (HPLC). TSS does not represent this product as USP, BP, EP, or any pharmacopoeia grade.
What are the safety and handling considerations for this chemical?
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This product requires the use of personal protective equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The WGK Germany classification is 3.
How is this chemical packed and shipped?
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The product is typically stored at -20°C. TSS ships and exports fine chemicals, pharmaceutical intermediates, and laboratory reagents globally. Specific packaging details are available upon request.
How can I request a sample or quote for 5-Bromo-4-chloro-3-indolyl phosphate disodium salt?
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To request a sample or quote, please contact our sales team through the website or via the provided contact information, specifying the product name and CAS number (102185-33-1).
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