5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (CAS 6578-06-9) is a chemical compound with a molecular weight of 433.62 g/mol. It serves as a chromogenic substrate for the detection of alkaline phosphatase activity. This reagent is vital in various molecular biology techniques, producing a distinct purple precipitate upon enzymatic cleavage. Its solubility characteristics favour specific laboratory applications.
- IUPAC
- BCIP® p-toluidine salt,X-phosphate p-toluidine salt
- Synonyms
- BCIP® p-toluidine saltX-phosphate p-toluidine salt
Colorimetric Detection of Alkaline Phosphatase
Used to detect alkaline phosphatase activity in biological assays. The enzymatic reaction produces a visible purple precipitate, facilitating the identification of enzyme presence.
Northern, Southern, and Western Blotting
Applied in blotting techniques for the detection of specific nucleic acid or protein targets labelled with alkaline phosphatase. It enables visualisation of results.
In Situ Hybridization
Utilised in locating specific DNA or RNA sequences within tissue sections. The substrate allows for the visual mapping of target molecules.
Immunohistochemistry
Employed in the detection of antigens in tissue samples. Alkaline phosphatase conjugates are visualised using this chromogenic substrate.
| Molecular weight | 433.62 |
|---|---|
| Empirical formula | C8H6BrClNO4P · C7H9N |
| Assay | ≥99% |
| Solubility | DMF: soluble20 mg/mLH2O: insoluble |
| Storage temperature | −20°C |
| Application | For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt used for?
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt is used as a chromogenic substrate for the colorimetric detection of alkaline phosphatase activity in various molecular biology applications such as blotting, in situ hybridization, and immunohistochemistry.
What is the CAS number and formula for 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt?
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The CAS number is 6578-06-9 and the empirical formula (Hill notation) is C8H6BrClNO4P · C7H9N.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt with an assay of ≥99%. We do not represent this product as USP, BP, EP, or other pharmacopoeia grades.
How should 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt be handled?
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Handle with appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, and gloves. Avoid inhalation of dust and contact with skin and eyes. The compound is an irritant to the respiratory system, skin, and eyes.
Is this product available for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt.
How can I request a sample or quote?
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Please contact our sales team directly through our website or by phone to request a sample or a quotation for 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt.
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