5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt
5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt (CAS: 102185-49-9) is a chromogenic substrate for alkaline phosphatase. Upon enzymatic hydrolysis, it yields a water-insoluble blue precipitate, facilitating detection in various biochemical assays. It is primarily utilised in molecular biology and diagnostics for applications such as Western blotting and immunohistochemistry. This high-purity reagent from TSS is supplied for laboratory research and development purposes.
- IUPAC
- BCIP®,X-phosphate dipotassium salt
- Synonyms
- BCIP dipotassium salt5-Bromo-4-chloro-3-indolyl phosphate 2-potassium salt
Alkaline Phosphatase Detection
Serves as a chromogenic substrate for alkaline phosphatase, producing a distinct blue colour upon cleavage. This property is invaluable for visualising enzyme activity in various biological samples and assays.
Western Blotting
A key reagent in Western blotting techniques for the detection of immobilized proteins. Its enzymatic reaction allows for the visualisation of specific protein bands after antibody binding and enzyme conjugation.
Immunohistochemistry
Used in immunohistochemistry to detect alkaline phosphatase activity in tissue sections. The resulting precipitate helps localise the enzyme within specific cellular structures or tissues.
Enzyme-Linked Assays
Applicable in various enzyme-linked immunoassays (ELISA) and other biochemical tests where alkaline phosphatase is employed as a reporter enzyme, enabling quantitative or qualitative detection.
| Molecular weight | 402.65 |
|---|---|
| Linear formula | C8H4BrClNO4PK2 |
| Storage temperature | −20°C |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt used for?
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This compound is a chromogenic substrate for alkaline phosphatase, widely used in techniques such as Western blotting and immunohistochemistry for detecting enzyme activity.
What are the CAS number and chemical formula?
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The CAS number is 102185-49-9 and the linear formula is C<sub>8</sub>H<sub>4</sub>BrClNO<sub>4</sub>PK<sub>2}.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research and development. The specific assay and purity are detailed in the product specifications. TSS does not represent this product as USP, BP, or EP grade.
What are the safety and handling considerations?
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This compound is classified under WGK Germany 3, indicating it is hazardous to water. Appropriate personal protective equipment should be used, and contact with skin and eyes avoided. Consult the Safety Data Sheet for full details.
How is this chemical packed, shipped, and exported?
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Tech Serve Solutions expertly packs and ships fine chemicals globally, adhering to all relevant international regulations for hazardous materials. We are experienced exporters to research institutions and industrial partners worldwide.
How can I request a sample or quote?
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Please contact our sales team or use the online request form on our website to inquire about samples or obtain a quotation for 5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt.
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