5-Bromo-3-indolyl β-D-galactopyranoside

CAS 97753-82-7C14H16BrNO6Biochemicals & Reagents

5-Bromo-3-indolyl β-D-galactopyranoside (CAS 97753-82-7) is a chromogenic substrate for beta-galactosidase. With a molecular weight of 374.18 g/mol, it is primarily used in microbiology for the identification of bacterial colonies expressing the lac+ phenotype. This compound produces a distinct dark blue colour upon enzymatic cleavage, offering an alternative to similar substrates like X-Gal.

IUPAC: Bluo-Gal
Synonyms: Bluo-Gal

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5-Bromo-3-indolyl β-D-galactopyranoside (C14H16BrNO6) — chemical structure, CAS 97753-82-7; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsEnzyme SubstratesEnzymesInhibitorsand SubstratesGalactosidasebetaSubstrates by Enzyme

01 /Applications

Bacterial Colony Identification

Serves as a chromogenic substrate for detecting beta-galactosidase activity in bacterial colonies. It is particularly useful for identifying lac+ strains, where the enzyme cleaves the substrate to yield a dark blue precipitate.

Microbiological Assays

A valuable reagent in microbiological research and diagnostics for visualising and differentiating bacterial populations based on their enzymatic profiles. Its darker blue colouration provides clear results.

Enzyme Substrate for β-Galactosidase

Specifically designed as a substrate for the enzyme beta-galactosidase (β-Gal). Its enzymatic hydrolysis leads to the formation of a coloured product, facilitating qualitative and quantitative assessments.

02 /Properties

Molecular weight	374.18
Empirical formula	C14H16BrNO6
Assay	≥98%
Solubility	DMF: soluble
Storage temperature	−20°C
Application	Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.
Suitability	suitable for substrate for β-galactosidase

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

97753-82-7

Beilstein registry

1550140

05 /Additional specifications

Sterility	non-sterile

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is 5-Bromo-3-indolyl β-D-galactopyranoside used for?

5-Bromo-3-indolyl β-D-galactopyranoside is used as a chromogenic substrate for the identification of bacterial colonies expressing the lac+ phenotype due to its enzymatic cleavage by beta-galactosidase, producing a dark blue colour.

What is the CAS number and formula for this compound?

The CAS number is 97753-82-7 and the empirical formula is C14H16BrNO6.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies 5-Bromo-3-indolyl β-D-galactopyranoside with an assay of ≥98%. This product is supplied for research and laboratory use and is not intended for USP, BP, EP, or other pharmacopoeial applications.

What are the safety and handling precautions for this chemical?

Handling requires personal protective equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The product carries a WGK Germany rating of 3, indicating it is hazardous to water.

How is this product typically packed, shipped, and exported?

This chemical is supplied non-sterile and is stored at −20°C. Tech Serve Solutions, a global supplier and exporter since 1998, handles all packing, shipping, and export logistics in compliance with international regulations for fine chemicals.

How can I request a quote or sample?

To request a quote or a sample of 5-Bromo-3-indolyl β-D-galactopyranoside, please contact our sales team through the website or by phone. We cater to global research and industrial clients.