4-Nitrophenyl β-D-glucopyranoside
4-Nitrophenyl β-D-glucopyranoside (CAS: 2492-87-7, C12H15NO8) is a chromogenic enzyme substrate supplied by Tech Serve Solutions. This compound, with a molecular weight of 301.25 g/mol, is primarily utilised in biochemical research for the detection and quantification of β-glucosidase activity. Its key application lies in enzymatic assays where its hydrolysis yields a coloured product, enabling spectrophotometric measurement.
- IUPAC
- p-Nitrophenyl β-D-glucopyranoside
- Synonyms
- p-Nitrophenyl beta-D-glucopyranoside4-Nitrophenyl beta-D-glucosidep-Nitrophenyl-beta-D-glucoside

Enzyme Activity Assays
Serves as a chromogenic substrate for the detection and measurement of β-glucosidase enzyme activity. Hydrolysis by the enzyme releases p-nitrophenol, which can be quantified spectrophotometrically, often around 400-410 nm, to determine enzyme kinetics and concentration.
Biochemical Research
Used in general biochemical studies, particularly in glycobiology and molecular biology, to investigate enzyme function and biological pathways involving glucose. It aids in understanding post-translational modifications and proteomic studies where glycosylation is a factor.
Diagnostic Kits
Can be incorporated into diagnostic kits for the rapid identification of microorganisms that produce β-glucosidase, an enzyme often associated with specific bacterial species. This aids in clinical and food safety microbiology.
| Molecular weight | 301.25 |
|---|---|
| Empirical formula | C12H15NO8 |
| Assay | ≥98% (TLC) |
| Storage temperature | −20°C |


Hazard statements
- H302Harmful if swallowed
- H371May cause damage to organs
Precautionary statements
- P260Do not breathe dust, fume, gas, mist, vapours or spray
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/21/22-68/20/21/22 |
| Safety statements (S) | 22-33-36/37-60 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl β-D-glucopyranoside used for?
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4-Nitrophenyl β-D-glucopyranoside is primarily used as a chromogenic substrate for the detection and quantification of β-glucosidase activity in biochemical research and diagnostic applications.
What are the CAS number and formula for 4-Nitrophenyl β-D-glucopyranoside?
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The CAS number is 2492-87-7, and the empirical formula is C12H15NO8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl β-D-glucopyranoside at a PREMIUM quality level with an assay of ≥98% (TLC). This product is not intended for human or veterinary use and is not represented as USP, BP, EP, or pharmaceutical grade.
How should 4-Nitrophenyl β-D-glucopyranoside be handled and stored?
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4-Nitrophenyl β-D-glucopyranoside is classified as harmful if swallowed (H302) and may cause damage to organs through prolonged or repeated exposure (H371). It should be handled with appropriate personal protective equipment in a well-ventilated area. Store at −20°C.
Is 4-Nitrophenyl β-D-glucopyranoside a controlled substance?
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Based on the provided data, 4-Nitrophenyl β-D-glucopyranoside is not listed as a controlled substance.
How is 4-Nitrophenyl β-D-glucopyranoside packed, shipped, and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, ensuring appropriate packaging and compliance with international shipping regulations for products like 4-Nitrophenyl β-D-glucopyranoside.
How can I request a sample or a quote for 4-Nitrophenyl β-D-glucopyranoside?
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To request a sample or a quote for 4-Nitrophenyl β-D-glucopyranoside, please contact the Tech Serve Solutions sales team through our official website or contact details.
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