4-Nitrophenyl β-D-lactopyranoside
4-Nitrophenyl β-D-lactopyranoside (CAS: 4419-94-7, C18H25NO13) is a biochemical reagent and enzyme substrate. It serves as a chromogenic substrate for detecting enzymatic activity, particularly in studies involving glycosidases. This compound is vital in research for investigating biological processes, including glycobiology and post-translational modifications. Its chemical structure facilitates the release of a coloured product upon enzymatic cleavage, enabling quantitative assays. TSS supplies this reagent for laboratory and research applications.

Enzyme Activity Assays
Used as a chromogenic substrate for the quantitative determination of enzyme activity, particularly β-galactosidase. Enzymatic hydrolysis releases 4-nitrophenol, which can be detected spectrophotometrically.
Glycobiology Research
A valuable tool in glycobiology for studying carbohydrate-protein interactions and enzymatic modifications of glycoproteins and glycolipids. It aids in understanding complex biological pathways.
Biochemical Research
Applicable in various biochemical studies requiring a sensitive and specific substrate for enzyme-linked reactions. Its use supports research in molecular biology and proteomics.
| Molecular weight | 463.39 |
|---|---|
| Empirical formula | C18H25NO13 |
| Form | powder |
| Colour | white |
| Storage temperature | −20°C |

Hazard statements
- H360D
Precautionary statements
- P201Obtain special instructions before use
- P308IF exposed or concerned
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | T |
| Risk statements (R) | 61 |
| Safety statements (S) | 53-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl β-D-lactopyranoside used for?
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4-Nitrophenyl β-D-lactopyranoside is primarily used as a chromogenic substrate for the detection and quantification of enzyme activity, particularly for enzymes like β-galactosidase, and in various glycobiology and biochemical research applications.
What is the CAS number and chemical formula?
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The CAS number for 4-Nitrophenyl β-D-lactopyranoside is 4419-94-7, and its chemical formula is C18H25NO13.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl β-D-lactopyranoside as a white powder for research purposes. The specific assay and purity are detailed in the product specifications. We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade unless explicitly stated.
How should 4-Nitrophenyl β-D-lactopyranoside be handled and stored?
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This compound is classified as Danger, with hazard statement H360D (May damage the unborn child). Handle with extreme caution in a well-ventilated area, using appropriate personal protective equipment. Store at -20°C as indicated.
Is 4-Nitrophenyl β-D-lactopyranoside a controlled substance?
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Based on the provided data, there is no indication that 4-Nitrophenyl β-D-lactopyranoside is a controlled substance. However, users should always verify local regulations.
How is this product packed and shipped?
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4-Nitrophenyl β-D-lactopyranoside is supplied as a white powder and shipped according to standard chemical handling protocols. Storage is recommended at -20°C. TSS is a global supplier and exporter of fine chemicals.
How can I request a sample or quote?
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To request a sample or a quote for 4-Nitrophenyl β-D-lactopyranoside, please contact our sales department through the TSS website or by phone. Our team will be pleased to assist you.
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