4-Nitrophenyl β-D-glucuronide

CAS 10344-94-2C12H13NO9Biochemicals & Reagents

4-Nitrophenyl β-D-glucuronide (CAS: 10344-94-2), with the molecular formula C12H13NO9 and a molecular weight of 315.23 g/mol, is a valuable biochemical reagent. It is specifically identified as a suitable substrate for the enzyme β-glucuronidase. This compound plays a crucial role in various enzymatic assays and biochemical research applications, particularly in diagnostics and enzyme activity studies.

IUPAC: 4-Nitrophenyl-β-D-glucopyranosiduronic acid,PNPG
Synonyms: 4-Nitrophenyl-β-D-glucopyranosiduronic acidPNPG

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4-Nitrophenyl β-D-glucuronide (C12H13NO9) — chemical structure, CAS 10344-94-2; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsEnzyme SubstratesEnzymesInhibitorsand SubstratesGlucuronidasebeta-Substrates by Enzyme

01 /Applications

Enzyme Substrate for β-Glucuronidase

This compound serves as a specific chromogenic substrate for the enzyme β-glucuronidase. Upon enzymatic hydrolysis, it releases 4-nitrophenol, which can be quantified spectrophotometrically, allowing for the determination of enzyme activity.

Diagnostic Assays

It is utilised in diagnostic kits and assays, particularly for detecting β-glucuronidase activity in biological samples. This is relevant in microbiology for identifying specific bacterial species or in clinical chemistry.

Biochemical Research

4-Nitrophenyl β-D-glucuronide is employed in academic and industrial research settings to study enzyme kinetics, inhibition, and other biochemical pathways involving glucuronidation or glucuronidase activity.

02 /Properties

Molecular weight	315.23
Empirical formula	C12H13NO9
Assay	≥99.0% (TLC)
Solubility	H2O: soluble0.1 g/mL, clear, faintly yellow
Optical activity	[α]20/D −102±3°, c = 1% in ethanol
Storage temperature	−20°C
Impurities tested	≤0.05% free 4-nitrophenol≤7% water
Suitability	suitable as substrate for β-glucuronidase test

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

10344-94-2

MDL number

MFCD00036210

Beilstein registry

1438576

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 4-Nitrophenyl β-D-glucuronide used for?

4-Nitrophenyl β-D-glucuronide is primarily used as a chromogenic substrate for the enzyme β-glucuronidase in biochemical assays and diagnostic testing. It is suitable for detecting and quantifying β-glucuronidase activity.

What are the CAS number and formula for this product?

The CAS number is 10344-94-2, and the empirical formula is C12H13NO9.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies 4-Nitrophenyl β-D-glucuronide with an assay of ≥99.0% (TLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.

How should 4-Nitrophenyl β-D-glucuronide be handled and stored?

This compound should be handled with appropriate personal protective equipment, including eyeshields, gloves, and a suitable respirator filter (type N95 or P1 EN143). It is classified under WGK Germany 3. Store at -20°C.

Is 4-Nitrophenyl β-D-glucuronide a controlled substance?

The provided data does not indicate that 4-Nitrophenyl β-D-glucuronide is a controlled substance.

How is this chemical packed, shipped, and exported?

Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. Products are packed and shipped according to international regulations for chemical transport. Please contact us for specific export inquiries.

How can I request a sample or quote?

To request a sample or a quote for 4-Nitrophenyl β-D-glucuronide, please contact our sales team via the website or direct email. Our experts will assist you with your requirements.