4-Nitrophenyl β-D-fucopyranoside
4-Nitrophenyl β-D-fucopyranoside (CAS: 1226-39-7), with the molecular formula C12H15NO7 and a molecular weight of 285.25 g/mol, is a biochemical reagent. It serves as a chromogenic substrate, primarily utilised in enzymatic assays to detect and quantify the activity of specific glycosidases. Its applications span across glycobiology research and molecular biology studies, aiding in the understanding of post-translational modifications and proteomic analyses.
Enzyme Substrate in Glycobiology
This compound acts as a synthetic substrate for beta-D-fucosidase, enabling researchers to study enzyme kinetics and mechanisms. The enzymatic cleavage releases 4-nitrophenol, which can be quantified spectrophotometrically, providing a sensitive method for enzyme activity measurement.
Chromogenic Detection in Biochemical Assays
Its chromogenic properties make it valuable for developing assays in molecular biology and proteomics. The release of the yellow coloured 4-nitrophenol upon enzymatic hydrolysis allows for straightforward visual or instrument-based detection of enzyme presence and activity.
Research in Post-Translational Modifications
4-Nitrophenyl β-D-fucopyranoside is employed in studies investigating the role of fucosylation in protein function and biological processes. It assists in understanding how glycosylation patterns are modified and their impact on cellular mechanisms.
| Molecular weight | 285.25 |
|---|---|
| Empirical formula | C12H15NO7 |
| Assay | ≥98% (TLC) |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl β-D-fucopyranoside used for?
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4-Nitrophenyl β-D-fucopyranoside is primarily used as a chromogenic substrate for the detection and quantification of beta-D-fucosidase activity in biochemical and molecular biology research, particularly in the fields of glycobiology and proteomics.
What is the CAS number and formula for 4-Nitrophenyl β-D-fucopyranoside?
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The CAS number for 4-Nitrophenyl β-D-fucopyranoside is 1226-39-7, and its molecular formula is C12H15NO7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl β-D-fucopyranoside with an assay of ≥98% (TLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeial grade.
How should 4-Nitrophenyl β-D-fucopyranoside be handled and stored?
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This compound requires careful handling. Personal protective equipment including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter should be used. It should be stored at -20°C.
Is 4-Nitrophenyl β-D-fucopyranoside a controlled substance?
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Based on the provided data, 4-Nitrophenyl β-D-fucopyranoside (CAS: 1226-39-7) is not listed as a controlled substance.
How can I request a quote or sample for 4-Nitrophenyl β-D-fucopyranoside?
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To request a quote or a sample of 4-Nitrophenyl β-D-fucopyranoside, please contact our sales department through the website or by phone. We are a global supplier and exporter.
What are the GHS hazards for 4-Nitrophenyl β-D-fucopyranoside?
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The provided safety data indicates a WGK (Germany) of 3, suggesting it is hazardous. Specific GHS hazard statements are not detailed, but standard laboratory precautions are advised.
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