4-Nitrophenyl β-D-cellobioside
4-Nitrophenyl β-D-cellobioside (CAS 3482-57-3) is a chemical compound with the formula C18H25NO13 and a molecular weight of 463.39 g/mol. As a chromogenic substrate, it is primarily utilised in biochemical research for the detection and quantification of enzyme activity, particularly cellulase and related glycosidases. This premium grade reagent is essential for studies in glycobiology, enzyme kinetics, and molecular biology.
- IUPAC
- p-Nitrophenyl β-D-cellobioside

Enzyme Activity Assays
Utilised as a chromogenic substrate to detect and quantify the activity of enzymes such as cellulase and β-glucosidase by measuring the release of the p-nitrophenol chromophore.
Glycobiology Research
A valuable tool in the study of carbohydrate structures and their metabolism, aiding investigations into glycosidic bond cleavage and enzyme function in biological systems.
Diagnostic Tool Development
Can be employed in the development of diagnostic assays for identifying specific enzyme deficiencies or microbial activities in various biological samples.
Biochemical Research Reagent
Serves as a high-quality research reagent for academic and industrial laboratories investigating enzyme mechanisms, substrate specificity, and related biochemical pathways.
| Molecular weight | 463.39 |
|---|---|
| Empirical formula | C18H25NO13 |
| Assay | ≥98% (TLC) |
| Storage temperature | 2-8°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl β-D-cellobioside used for?
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4-Nitrophenyl β-D-cellobioside is used as a chromogenic substrate in biochemical research to detect and quantify the activity of enzymes, such as cellulase and related glycosidases, by measuring the release of p-nitrophenol.
What is the CAS number and formula for 4-Nitrophenyl β-D-cellobioside?
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The CAS number is 3482-57-3 and the chemical formula is C18H25NO13.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product at a PREMIUM quality level with an assay of ≥98% (TLC). We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
What are the safety and handling recommendations for 4-Nitrophenyl β-D-cellobioside?
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It requires the use of safety equipment including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The WGK Germany classification is 3, indicating a high hazard to water.
How is 4-Nitrophenyl β-D-cellobioside shipped and exported?
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This product is typically stored at 2-8°C. Tech Serve Solutions is a specialist global supplier and exporter, adhering to all relevant regulations for the safe and compliant shipment of chemicals worldwide.
How can I request a sample or a quote for 4-Nitrophenyl β-D-cellobioside?
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Please contact our sales team directly through our website or by phone to request a sample or obtain a quotation for 4-Nitrophenyl β-D-cellobioside.
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