4-Nitrophenyl β-D-galactopyranoside
4-Nitrophenyl β-D-galactopyranoside (CAS: 3150-24-1; Formula: C12H15NO8; MW: 301.25) is a premium biochemical reagent. This chromogenic substrate is primarily used for the detection and quantification of β-galactosidase enzyme activity. Its reliable performance makes it an essential tool in molecular biology, glycobiology, and proteomics research for various enzymatic assays and diagnostics.
- IUPAC
- p-Nitrophenyl β-D-galactopyranoside

Enzyme Activity Assays
Serves as a chromogenic substrate for quantifying β-galactosidase activity. Upon enzymatic cleavage, it releases p-nitrophenol, which can be detected spectrophotometrically, enabling precise enzyme activity measurements.
Molecular Biology Research
Widely employed in molecular biology for detecting lacZ gene expression, a common reporter gene in genetic engineering. This allows researchers to monitor gene transcription and protein expression in various experimental systems.
Biochemical Research
Useful in glycobiology and proteomics for studying enzyme kinetics and substrate specificity related to galactosidase enzymes. It aids in understanding post-translational modifications and protein function.
| Molecular weight | 301.25 |
|---|---|
| Empirical formula | C12H15NO8 |
| Assay | ≥98% (TLC)≥98% (enzymatic) |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl β-D-galactopyranoside used for?
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4-Nitrophenyl β-D-galactopyranoside is used as a chromogenic substrate for detecting and quantifying β-galactosidase enzyme activity in biochemical and molecular biology research.
What are the CAS number and formula for 4-Nitrophenyl β-D-galactopyranoside?
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The CAS number is 3150-24-1 and the molecular formula is C12H15NO8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl β-D-galactopyranoside at a PREMIUM quality level with an assay of ≥98% (TLC) and ≥98% (enzymatic). This product is not represented as USP, BP, or EP grade.
How should 4-Nitrophenyl β-D-galactopyranoside be handled?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used. The substance is classified with WGK Germany 3, indicating a high hazard to water.
How is 4-Nitrophenyl β-D-galactopyranoside shipped and exported?
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Tech Serve Solutions, established in 1998, specialises in the global supply and export of fine chemicals, including 4-Nitrophenyl β-D-galactopyranoside, adhering to all necessary shipping regulations.
How can I request a sample or quote for 4-Nitrophenyl β-D-galactopyranoside?
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To request a sample or a quote, please contact our sales team through the TSS website or via direct inquiry, providing your specific requirements and delivery details.
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