4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-α-D-galactopyranoside
4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-α-D-galactopyranoside (CAS: 125455-64-3) is a complex carbohydrate derivative with a molecular weight of 545.49. This compound serves as a valuable biochemical reagent, particularly recognised as a chromogenic enzyme substrate. Its specific structure allows for the detection and study of enzymatic activity in various biological and biochemical research contexts.
![4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-α-D-galactopyranoside ((β-D-GlcNAc-[1→3]-α-D-GalNAc-1→OC6H4NO2) — chemical structure, CAS 125455-64-3; Life Science, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F125455-64-3.png&w=3840&q=75)
Enzyme Substrate in Biochemical Assays
This compound functions as a specific substrate for certain glycosidases, enabling the detection of enzymatic activity through a chromogenic reaction. It is widely employed in biochemical research to quantify enzyme kinetics and screen for inhibitors.
Glycobiology Research Tool
Its well-defined disaccharide structure makes it useful in studies related to glycobiology, post-translational modifications, and proteomics. Researchers utilise it to investigate carbohydrate-protein interactions and enzymatic processing of glycoproteins.
Chromogenic Reagent
The presence of the 4-nitrophenyl group facilitates a colorimetric readout upon enzymatic cleavage. This property makes it a convenient tool for developing assays where a visual or spectrophotometric signal is required.
| Molecular weight | 545.49 |
|---|---|
| Linear formula | (β-D-GlcNAc-[1→3]-α-D-GalNAc-1→OC6H4NO2 |
| Assay | ≥98% |
| Storage temperature | −20°C |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 22-26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-α-D-galactopyranoside used for?
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This compound is primarily used as a chromogenic enzyme substrate in biochemical assays and glycobiology research. It helps in detecting and quantifying enzyme activity, studying post-translational modifications, and investigating carbohydrate-protein interactions.
What are the CAS number and formula for this compound?
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The CAS number is 125455-64-3. The linear formula provided is (β-<sc>D</sc>-GlcNAc-[1→3]-α-<sc>D</sc>-GalNAc-1→OC<sub>6</sub>H<sub>4</sub>NO<sub>2</sub>).
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥98%. We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade, and it is intended for research and laboratory use.
How should this chemical be handled and stored?
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This compound is a Warning irritant (H315, H319, H335). Handle with appropriate personal protective equipment, including a dust mask (N95), eyeshields, and gloves. It should be stored at −20°C.
Is this chemical shipped internationally?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents, and can export this product worldwide.
How can I request a quote or sample?
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To request a quote or a sample, please contact our sales team through the website or by phone. Our team will be happy to assist you with your inquiry.
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