4-Nitrophenyl 4-guanidinobenzoate hydrochloride

CAS 19135-17-2C14H12N4O4 · HClBiochemicals & Reagents

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (CAS: 19135-17-2) is a biochemical reagent with the molecular formula C14H12N4O4 · HCl and a molecular weight of 336.73 g/mol. It serves as a chromogenic substrate, primarily utilised in enzymatic assays. This compound is frequently employed in research settings for its ability to facilitate the detection and quantification of enzyme activity, particularly in studies involving plasma and blood proteins.

IUPAC: 4-Guanidinobenzoic acid 4-nitrophenylester hydrochloride,NPGB,pNPGB
Synonyms: 4-Guanidinobenzoic acid 4-nitrophenylester hydrochloride,NPGB,pNPGB

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4-Nitrophenyl 4-guanidinobenzoate hydrochloride (C14H12N4O4 · HCl) — chemical structure, CAS 19135-17-2; Life Science, fine chemical supplied by Tech Serve Solutions

ActivityBiochemicals and ReagentsChromogenicEnzyme SubstratesEnzymesInhibitorsand SubstratesPlasma & Blood ProteinsPlasminProteins and Derivatives

01 /Applications

Enzyme Substrate

4-Nitrophenyl 4-guanidinobenzoate hydrochloride is employed as a chromogenic substrate in enzymatic assays. It allows for the spectrophotometric detection and quantification of enzyme activity by releasing 4-nitrophenol upon cleavage.

Biochemical Research

This compound is a valuable tool in biochemical research, particularly for studying enzymes involved in protein hydrolysis and blood coagulation pathways. It aids in understanding enzyme kinetics and inhibition.

Plasmin Assays

It is specifically used as a substrate for plasmin and other serine proteases, enabling the accurate assessment of their activity levels in biological samples.

02 /Properties

Molecular weight	336.73
Empirical formula	C14H12N4O4 · HCl
Assay	≥98% (TLC)
Storage temperature	−20°C

03 /Safety & handling

GHS hazard pictogram: Corrosive (GHS05) — Corrosive

Danger

Hazard statements

H318Causes serious eye damage

Precautionary statements

P280Wear protective gloves, clothing and eye/face protection
P305IF IN EYES

Protective equipment	dust mask type N95 (US), Eyeshields, Gloves
Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xi
Risk statements (R)	36
Safety statements (S)	26

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

19135-17-2

MDL number

MFCD00037010

PubChem substance

24897847

Beilstein registry

8024554

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 4-Nitrophenyl 4-guanidinobenzoate hydrochloride used for?

4-Nitrophenyl 4-guanidinobenzoate hydrochloride is primarily used as a chromogenic substrate in biochemical research for the detection and quantification of enzyme activity, particularly for enzymes like plasmin.

What is the CAS number and molecular formula?

The CAS number for 4-Nitrophenyl 4-guanidinobenzoate hydrochloride is 19135-17-2, and its molecular formula is C14H12N4O4 · HCl.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies 4-Nitrophenyl 4-guanidinobenzoate hydrochloride with an assay of ≥98% as determined by TLC. It is supplied for research and laboratory use and is not represented as USP, BP, or EP grade.

What are the safety and handling precautions?

This compound can cause serious eye damage (H318). Handle with a dust mask (type N95 US), eyeshields, and gloves. Ensure good ventilation. In case of contact with eyes, rinse cautiously with water for several minutes and seek medical advice.