4-Nitrophenyl N-acetyl-α-D-glucosaminide
4-Nitrophenyl N-acetyl-α-D-glucosaminide (CAS: 10139-02-3) is a biochemical reagent with the molecular formula C14H18N2O8 and a molecular weight of 342.30 g/mol. This compound serves as a chromogenic enzyme substrate, particularly for enzymes involved in the hydrolysis of N-acetyl-α-D-glucosaminide linkages. It is widely utilized in biochemical research and diagnostics for enzyme activity assays.
- IUPAC
- p-Nitrophenyl 2-acetamido-2-deoxy-α-D-glucopyranoside
- Synonyms
- p-Nitrophenyl 2-acetamido-2-deoxy-α-D-glucopyranoside

Enzyme Activity Assays
Used as a chromogenic substrate to quantify the activity of enzymes that cleave N-acetyl-α-D-glucosaminide bonds, such as glycosidases. Upon enzymatic hydrolysis, the p-nitrophenol moiety is released, producing a yellow colour measurable spectrophotometrically.
Glycobiology Research
A valuable tool in glycobiology for studying carbohydrate metabolism, enzyme kinetics, and the structure-function relationships of glycoproteins and glycolipids. It aids in understanding cellular processes involving N-acetylglucosamine.
Diagnostic Applications
Can be employed in diagnostic kits for the detection of specific enzyme activities in biological samples, contributing to the diagnosis of certain metabolic disorders or infections.
Biochemical Research Reagent
Serves as a standard reagent in molecular biology and proteomics research, particularly for investigations into post-translational modifications and enzyme-substrate interactions.
| Molecular weight | 342.30 |
|---|---|
| Empirical formula | C14H18N2O8 |
| Assay | ≥98% |
| Storage temperature | −20°C |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl N-acetyl-α-D-glucosaminide used for?
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4-Nitrophenyl N-acetyl-α-D-glucosaminide (CAS: 10139-02-3) is primarily used as a chromogenic substrate for enzyme activity assays, especially for enzymes hydrolyzing N-acetyl-α-D-glucosaminide linkages. It is also valuable in glycobiology and biochemical research.
What is the CAS number and formula for 4-Nitrophenyl N-acetyl-α-D-glucosaminide?
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The CAS number is 10139-02-3 and the empirical formula is C14H18N2O8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl N-acetyl-α-D-glucosaminide with an assay of ≥98%. TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade.
What are the safety and handling recommendations for this compound?
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Based on its classification (WGK Germany: 3), this compound should be handled with care. Refer to the Safety Data Sheet (SDS) for detailed information on hazards, personal protective equipment, and safe handling procedures.
Is this product available for export?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, and we can arrange for the shipment and export of 4-Nitrophenyl N-acetyl-α-D-glucosaminide.
How can I request a sample or quote for 4-Nitrophenyl N-acetyl-α-D-glucosaminide?
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Please contact our sales team directly via our website or by telephone to request a sample or obtain a quotation for 4-Nitrophenyl N-acetyl-α-D-glucosaminide.
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