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4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside

CAS 7284-16-4C22H31N3O13Biochemicals & Reagents

4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside (CAS: 7284-16-4) is a chromogenic substrate with a molecular weight of 545.49 g/mol. This biochemical reagent is primarily utilised in enzymatic assays for the detection and quantification of enzyme activity, particularly related to chitinases. Its well-defined structure allows for precise monitoring in biochemical research and diagnostic applications, serving as a valuable tool in glycobiology and molecular biology studies.

Synonyms
4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-N-acetyl-beta-D-glucosaminyl-(1->4)-2,3,6-tri-O-acetyl-beta-D-glucoside
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4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside (C22H31N3O13) — chemical structure, CAS 7284-16-4; Life Science, fine chemical supplied by Tech Serve Solutions
Biochemicals and ReagentsChitinaseChitobiosideChromogenicEnzyme SubstratesEnzymesInhibitorsand SubstratesGlycobiologyInhibitors and Substrates
01 /Applications

Enzyme Substrate for Chitinase Assays

This compound functions as a chromogenic substrate for the enzyme chitinase. Upon enzymatic cleavage, it releases a coloured product, enabling the quantitative assessment of chitinase activity in various biological samples and research settings.

Biochemical Research

Utilised in studies related to glycobiology, enzyme kinetics, and post-translational modifications. It aids researchers in understanding carbohydrate metabolism and the function of enzymes involved in the breakdown of chitinous materials.

Molecular Biology and Proteomics

Serves as a useful reagent in molecular biology laboratories for enzyme characterisation and in proteomics for investigating enzyme-substrate interactions within complex biological systems.

02 /Properties
Molecular weight545.49
Empirical formulaC22H31N3O13
Assay≥99% (TLC)
Storage temperature2-8°C
03 /Safety & handling
Protective equipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry
CAS number
7284-16-4
MDL number
MFCD00133738
PubChem substance
24897770
Beilstein registry
1445509
05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside used for?

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4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside is primarily used as a chromogenic substrate in enzymatic assays, particularly for the detection and quantification of chitinase activity. It is a valuable reagent in biochemical research, glycobiology, and molecular biology.

What are the CAS number and formula for 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside?

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The CAS number is 7284-16-4, and its empirical formula is C22H31N3O13.

What grade and purity does Tech Serve Solutions supply?

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Tech Serve Solutions supplies 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside with an assay of ≥99% (TLC). TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade; it is intended for research and laboratory use.

How should 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside be handled and stored?

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Handle this compound with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It should be stored at 2-8°C. The substance has a WGK Germany rating of 3, indicating it is hazardous to water.

Is this product suitable for pharmaceutical manufacturing?

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Based on the provided data, this product is supplied for research and laboratory use with an assay of ≥99% (TLC) and is not represented as a pharmacopoeia grade. Its suitability for pharmaceutical manufacturing would require further specific qualification and testing.

How can I request a quote or sample?

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Please contact the TSS sales team directly via the website or by phone to request a quotation or a sample of 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside.

How is this chemical shipped?

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Tech Serve Solutions, as a global supplier and exporter, handles the packing and shipping of chemicals like 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside according to international regulations for laboratory reagents and biochemicals, ensuring safe transit.

Need 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside in a specific grade or volume?