4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside
4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside (CAS: 7284-16-4) is a chromogenic substrate with a molecular weight of 545.49 g/mol. This biochemical reagent is primarily utilised in enzymatic assays for the detection and quantification of enzyme activity, particularly related to chitinases. Its well-defined structure allows for precise monitoring in biochemical research and diagnostic applications, serving as a valuable tool in glycobiology and molecular biology studies.
- Synonyms
- 4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-N-acetyl-beta-D-glucosaminyl-(1->4)-2,3,6-tri-O-acetyl-beta-D-glucoside

Enzyme Substrate for Chitinase Assays
This compound functions as a chromogenic substrate for the enzyme chitinase. Upon enzymatic cleavage, it releases a coloured product, enabling the quantitative assessment of chitinase activity in various biological samples and research settings.
Biochemical Research
Utilised in studies related to glycobiology, enzyme kinetics, and post-translational modifications. It aids researchers in understanding carbohydrate metabolism and the function of enzymes involved in the breakdown of chitinous materials.
Molecular Biology and Proteomics
Serves as a useful reagent in molecular biology laboratories for enzyme characterisation and in proteomics for investigating enzyme-substrate interactions within complex biological systems.
| Molecular weight | 545.49 |
|---|---|
| Empirical formula | C22H31N3O13 |
| Assay | ≥99% (TLC) |
| Storage temperature | 2-8°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside used for?
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4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside is primarily used as a chromogenic substrate in enzymatic assays, particularly for the detection and quantification of chitinase activity. It is a valuable reagent in biochemical research, glycobiology, and molecular biology.
What are the CAS number and formula for 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside?
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The CAS number is 7284-16-4, and its empirical formula is C22H31N3O13.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside with an assay of ≥99% (TLC). TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade; it is intended for research and laboratory use.
How should 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside be handled and stored?
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Handle this compound with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It should be stored at 2-8°C. The substance has a WGK Germany rating of 3, indicating it is hazardous to water.
Is this product suitable for pharmaceutical manufacturing?
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Based on the provided data, this product is supplied for research and laboratory use with an assay of ≥99% (TLC) and is not represented as a pharmacopoeia grade. Its suitability for pharmaceutical manufacturing would require further specific qualification and testing.
How can I request a quote or sample?
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Please contact the TSS sales team directly via the website or by phone to request a quotation or a sample of 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside.
How is this chemical shipped?
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Tech Serve Solutions, as a global supplier and exporter, handles the packing and shipping of chemicals like 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside according to international regulations for laboratory reagents and biochemicals, ensuring safe transit.
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