4-Nitrophenyl 1-thio-β-D-galactopyranoside
4-Nitrophenyl 1-thio-β-D-galactopyranoside (CAS: 1230-27-9; Formula: C12H15NO7S) is a synthetic organic compound with a molecular weight of 317.32 g/mol. It serves as a chromogenic substrate, primarily utilised in biochemical research for the detection and assay of enzyme activity. Its structure allows for the release of a coloured product upon enzymatic cleavage, facilitating quantitative analysis in various biological and diagnostic applications.
Enzyme Substrate
This compound functions as a chromogenic substrate for detecting enzyme activity, particularly those enzymes that cleave galactosidic bonds. Upon enzymatic hydrolysis, it releases 4-nitrophenol, which is intensely yellow at alkaline pH, allowing for spectrophotometric quantification.
Biochemical Assays
Used in biochemical laboratories for the quantitative determination of enzyme kinetics and activity. It is valuable in studies involving glycosidases and related enzymes due to the straightforward detection method it enables.
Diagnostic Research
Its ability to produce a visible signal upon enzymatic action makes it a useful reagent in research settings for developing diagnostic assays. This can aid in identifying the presence or activity level of specific enzymes linked to biological processes.
| Molecular weight | 317.32 |
|---|---|
| Empirical formula | C12H15NO7S |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl 1-thio-β-D-galactopyranoside used for?
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4-Nitrophenyl 1-thio-β-D-galactopyranoside (CAS: 1230-27-9) is primarily used as a chromogenic substrate in biochemical assays to detect and quantify enzyme activity, particularly glycosidases.
What is the CAS number and chemical formula?
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The CAS number is 1230-27-9, and the chemical formula is C12H15NO7S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl 1-thio-β-D-galactopyranoside for research and laboratory use. Specific assay details are available on the product's Certificate of Analysis. We do not represent this product as USP, BP, or EP grade.
How should 4-Nitrophenyl 1-thio-β-D-galactopyranoside be handled?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A respirator filter like type N95 (US) or P1 (EN143) is recommended. Refer to the Safety Data Sheet for full handling guidelines, noting its WGK Germany classification of 3.
How is this chemical packed, shipped, and exported?
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This chemical is typically stored at -20°C. Tech Serve Solutions professionally packs, ships, and exports fine chemicals globally, adhering to all relevant transport regulations. Please contact us for specific shipping and export inquiries.
How can I request a sample or a quote?
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To request a sample or obtain a quote for 4-Nitrophenyl 1-thio-β-D-galactopyranoside, please use the 'Request a Quote' or 'Contact Us' form on our website, providing the product name and CAS number (1230-27-9).
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