4-Nitrophenyl N-acetyl-β-D-glucosaminide
4-Nitrophenyl N-acetyl-β-D-glucosaminide (CAS: 3459-18-5) is a premium biochemical reagent with the molecular formula C14H18N2O8 and a molecular weight of 342.30 g/mol. This compound serves as a vital chromogenic substrate, primarily utilised in enzymatic assays for the detection and quantification of β-N-acetylglucosaminidase activity. Its reliable performance makes it a staple in biochemical research, diagnostics, and glycobiology studies for analysing enzyme kinetics and function.
- IUPAC
- 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside
Enzyme Substrate for β-N-acetylglucosaminidase
4-Nitrophenyl N-acetyl-β-D-glucosaminide is a specific chromogenic substrate for the enzyme β-N-acetylglucosaminidase. Upon enzymatic hydrolysis, it releases p-nitrophenol, which can be detected spectrophotometrically, allowing for the quantitative assay of enzyme activity.
Glycobiology Research
This compound is instrumental in glycobiology for studying the structure, function, and metabolism of carbohydrates. It aids in understanding the role of N-acetylglucosaminidase in various biological processes, including cell signalling and development.
Diagnostic Assays
It can be employed in the development of diagnostic kits for detecting enzyme deficiencies or abnormalities related to β-N-acetylglucosaminidase, which are sometimes associated with certain genetic disorders.
Biochemical Research Tool
As a high-purity reagent, it is broadly used in general biochemical research for enzyme characterisation, inhibitor screening, and studying post-translational modifications involving N-acetylglucosamine.
| Molecular weight | 342.30 |
|---|---|
| Empirical formula | C14H18N2O8 |
| Assay | ≥99% (TLC) |
| Storage temperature | −20°C |
| Impurities tested | ≤0.05% Free p-nitrophenol |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl N-acetyl-β-D-glucosaminide used for?
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4-Nitrophenyl N-acetyl-β-D-glucosaminide is primarily used as a chromogenic substrate in enzymatic assays to detect and quantify the activity of β-N-acetylglucosaminidase. It is also valuable in glycobiology research, diagnostic assay development, and general biochemical studies.
What are the CAS number and molecular formula for 4-Nitrophenyl N-acetyl-β-D-glucosaminide?
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The CAS number is 3459-18-5 and the molecular formula is C14H18N2O8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl N-acetyl-β-D-glucosaminide at a PREMIUM quality level with an assay of ≥99% by TLC. We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
What are the safety and handling requirements for this compound?
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This compound requires careful handling. Recommended personal protective equipment includes eyeshields and gloves. A respirator filter of type N95 (US) or P1 (EN143) is advised. It is classified with a WGK Germany rating of 3, indicating a high hazard to water.
How is 4-Nitrophenyl N-acetyl-β-D-glucosaminide typically shipped and stored?
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For maintaining its stability and quality, 4-Nitrophenyl N-acetyl-β-D-glucosaminide is stored at -20°C. TSS ensures appropriate packaging for export to maintain product integrity during transit.
How can I request a sample or a quote for 4-Nitrophenyl N-acetyl-β-D-glucosaminide?
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To request a sample or a quotation for 4-Nitrophenyl N-acetyl-β-D-glucosaminide, please contact our sales team through the website or by calling us directly. Provide your specific requirements for prompt assistance.
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