4-Nitrophenyl butyrate
4-Nitrophenyl butyrate (CAS 2635-84-9), with the molecular formula C10H11NO4 and a molecular weight of 209.20 g/mol, is a chemical compound utilised primarily as an enzyme substrate and chromogen in biochemical research. It serves as a valuable reagent for detecting enzymatic activity, particularly esterase or lipase activity, by producing a coloured product upon cleavage. Its properties make it suitable for various laboratory applications.
- IUPAC
- p-Nitrophenyl butyrate,Butyric acid 4-nitrophenyl ester
- Synonyms
- p-Nitrophenyl butyrateButyric acid 4-nitrophenyl ester

Enzyme Substrate
4-Nitrophenyl butyrate acts as a chromogenic substrate for esterases and lipases. Upon enzymatic hydrolysis, it releases 4-nitrophenol, which can be quantified spectrophotometrically, allowing for the determination of enzyme activity.
Biochemical Reagent
This compound is employed in biochemical assays and studies involving lipid metabolism and enzyme kinetics. Its use as a chromogen aids in visualising and measuring enzymatic reactions in a laboratory setting.
Research Tool
As a fine chemical, 4-Nitrophenyl butyrate is used in academic and industrial research for developing and validating new assay methods or for investigating enzyme mechanisms.
| Molecular weight | 209.20 |
|---|---|
| Empirical formula | C10H11NO4 |
| Assay | ≥98% |
| Density | 1.19 g/mL at 20 °C(lit.) |
| Storage temperature | −20°C |

Hazard statements
- H317May cause an allergic skin reaction
Precautionary statements
- P280Wear protective gloves, clothing and eye/face protection
| Protective equipment | Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 43 |
| Safety statements (S) | 36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitrophenyl butyrate used for?
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4-Nitrophenyl butyrate is primarily used as a chromogenic substrate for detecting esterase and lipase activity in biochemical research and assays.
What are the CAS number and formula for 4-Nitrophenyl butyrate?
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The CAS number for 4-Nitrophenyl butyrate is 2635-84-9, and its empirical formula (Hill notation) is C10H11NO4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitrophenyl butyrate with an assay of ≥98%. We do not represent this product as USP, BP, EP, or any pharmacopoeial grade.
How should 4-Nitrophenyl butyrate be handled safely?
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4-Nitrophenyl butyrate is classified as a Warning substance (GHS07) with hazard statement H317 (May cause an allergic skin reaction). Appropriate personal protective equipment, including eyeshields, gloves, and suitable respiratory protection, should be used. Always consult the Safety Data Sheet (SDS) before handling.
Is 4-Nitrophenyl butyrate exported by Tech Serve Solutions?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including 4-Nitrophenyl butyrate.
How can I request a quote or sample for 4-Nitrophenyl butyrate?
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To request a quote or sample for 4-Nitrophenyl butyrate, please contact our sales team through the website or by phone.
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