4-Methylumbelliferyl oleate
4-Methylumbelliferyl oleate (CAS 18323-58-5), with the molecular formula C28H40O4 and a molecular weight of 440.61 g/mol, is a crucial fluorogenic substrate. It is specifically employed for the detection and assay of lipase activity. This compound is recognised for its utility in fluorescence-based detection methods within biochemical and enzymatic studies.
- IUPAC
- Oleic acid 4-methylumbelliferyl ester
- Synonyms
- Oleic acid 4-methylumbelliferyl ester

Lipase Activity Assay
4-Methylumbelliferyl oleate serves as a sensitive fluorogenic substrate for quantifying lipase enzyme activity. Upon enzymatic hydrolysis, it releases fluorescent umbelliferone, allowing for precise measurement of enzyme kinetics.
Biochemical Research
This compound is valuable in biochemical research for studying lipid metabolism and enzyme mechanisms. Its fluorescent properties make it suitable for in vitro assays and screening for enzyme inhibitors.
Fluorescence Spectroscopy
The compound's inherent fluorescence characteristics, with distinct excitation and emission wavelengths, make it suitable for fluorescence spectroscopy applications in analytical and diagnostic settings.
| Molecular weight | 440.61 |
|---|---|
| Empirical formula | C28H40O4 |
| Assay | ≥95% (HPCE) |
| Solubility | 2-methoxyethanol: solubleDMSO: solublechloroform: soluble |
| Storage temperature | −20°C |
| Application | suitable as fluorogenic substrate for lipases |
| Suitability | suitable for fluorescence |
| Melting point | 37-38 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 312 nm in methanolλex 327 nm; λem 449 nm in 0.1 M phosphate pH 7.0 (lipase) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Methylumbelliferyl oleate used for?
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4-Methylumbelliferyl oleate is used as a fluorogenic substrate for the detection and assay of lipase activity in biochemical and enzymatic studies.
What are the CAS number and formula for 4-Methylumbelliferyl oleate?
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The CAS number is 18323-58-5, and its molecular formula is C28H40O4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Methylumbelliferyl oleate with an assay of ≥95% (HPCE). This product is intended for research and laboratory use; TSS does not represent it as USP, BP, or EP grade.
What are the safety and handling precautions for 4-Methylumbelliferyl oleate?
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Handling 4-Methylumbelliferyl oleate requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. It is classified under WGK Germany 3, indicating a high hazard level.
How is 4-Methylumbelliferyl oleate shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, capable of shipping and exporting 4-Methylumbelliferyl oleate worldwide.
How can I request a sample or quote for 4-Methylumbelliferyl oleate?
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To request a sample or a quotation for 4-Methylumbelliferyl oleate, please contact our sales team via the website or direct inquiry.
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