4-Methylumbelliferyl butyrate
4-Methylumbelliferyl butyrate (CAS: 17695-46-4; C14H14O4) is a valuable biochemical reagent with a molecular weight of 246.26 g/mol. This fluorogenic substrate is primarily utilised for the detection and quantification of esterase and lipase activity. Its ability to emit fluorescence upon enzymatic hydrolysis makes it an effective tool in biochemical assays and spectroscopic studies. Supplied by Tech Serve Solutions, this compound adheres to stringent quality standards for research applications.
- IUPAC
- MU-Bu
Enzyme Activity Assays
4-Methylumbelliferyl butyrate serves as a fluorogenic substrate for the detection and measurement of esterase and lipase enzyme activity. Upon hydrolysis by these enzymes, it releases 4-methylumbelliferone, which fluoresces under UV light, allowing for sensitive quantification.
Spectroscopic Detection
The compound is suitable for fluorescence-based detection methods. Its characteristic fluorescence properties, particularly of the hydrolysis product, enable its use in various spectroscopic analytical techniques, including enzyme kinetics studies.
Biochemical Research
As a biochemical reagent, 4-Methylumbelliferyl butyrate is employed in fundamental research to study enzyme mechanisms, inhibitor screening, and cellular processes involving esterase and lipase functions.
| Molecular weight | 246.26 |
|---|---|
| Empirical formula | C14H14O4 |
| Assay | ≥95% (HPCE) |
| Solubility | DMF: solubleDMSO: soluble |
| Storage temperature | −20°C |
| Application | suitable as fluorogenic substrate for esterases/lipases |
| Suitability | suitable for fluorescence |
| Melting point | 90-92 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 312 nm in methanolλex 360 nm; λem 449 nm (Reaction product)λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Methylumbelliferyl butyrate used for?
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4-Methylumbelliferyl butyrate is used as a fluorogenic substrate for the detection and quantification of esterase and lipase enzyme activity, and in fluorescence-based spectroscopic detection methods for biochemical research.
What are the CAS number and molecular formula for 4-Methylumbelliferyl butyrate?
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The CAS number for 4-Methylumbelliferyl butyrate is 17695-46-4, and its molecular formula is C14H14O4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Methylumbelliferyl butyrate with an assay of ≥95% (HPCE). We do not represent this product as USP, BP, or EP grade.
What are the safety and handling considerations for 4-Methylumbelliferyl butyrate?
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According to GHS information, appropriate personal protective equipment includes eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The compound is classified with WGK Germany rating 3, indicating a high level of hazard in water. Always consult the Safety Data Sheet (SDS) before handling.
How is 4-Methylumbelliferyl butyrate stored and shipped?
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4-Methylumbelliferyl butyrate should be stored at -20°C. Tech Serve Solutions exports this chemical globally, adhering to all relevant shipping regulations for chemical compounds.
How can I request a sample or quote for 4-Methylumbelliferyl butyrate?
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To request a sample or a quote for 4-Methylumbelliferyl butyrate, please contact our sales team through the website or by calling our office. We will be pleased to assist you.
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