4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate
4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate (CAS: 34197-46-1) is a fluorescent enzyme substrate with the empirical formula C18H15N3O4 · HCl · xH2O. It is primarily utilised in biochemical research for the detection and quantification of enzyme activity, particularly proteases. This compound serves as a sensitive tool for studying enzyme kinetics and screening for enzyme inhibitors. Its fluorescent properties allow for high-throughput assays.
- IUPAC
- 4-Guanidinobenzoic acid 4-methylumbelliferyl ester hydrochloride,MUGB
- Synonyms
- 4-Guanidinobenzoic acid 4-methylumbelliferyl ester hydrochloride,MUGB
Enzyme Activity Detection
This compound functions as a chromogenic/fluorogenic substrate for various enzymes, enabling the detection and measurement of their catalytic activity. Upon enzymatic cleavage, it releases a fluorescent product (4-methylumbelliferone), allowing for sensitive quantification.
Protease Assays
It is specifically employed in assays for serine proteases such as Factor Xa and thrombin. The hydrolysis of the guanidinobenzoate ester bond by these enzymes leads to the release of fluorescent 4-methylumbelliferone.
Biochemical Research Tool
Used in molecular biology and biochemistry laboratories for the study of enzyme mechanisms, kinetics, and for screening potential enzyme inhibitors in drug discovery processes.
Fluorescent Spectroscopy
The inherent fluorescence of the released product makes it suitable for spectroscopic analysis, allowing for high-sensitivity detection and quantification in various biological samples.
| Molecular weight | 373.79 (anhydrous basis) |
|---|---|
| Empirical formula | C18H15N3O4 · HCl · xH2O |
| Assay | ≥85% (HPLC) |
| Storage temperature | 2-8°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 323 nm; λem 464 nm (solvent pH 6.0)λex 360 nm; λem 449 nm (Reaction Product) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate used for?
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It is used as a fluorescent enzyme substrate, primarily for detecting and quantifying the activity of serine proteases like Factor Xa and thrombin in biochemical research.
What are the CAS number and formula for 4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate?
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The CAS number is 34197-46-1, and the formula is C18H15N3O4 · HCl · xH2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥85% by HPLC. We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety and handling recommendations for this product?
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According to GHS classifications, appropriate personal protective equipment includes eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It is classified with WGK Germany rating 3.
How is this chemical shipped and exported?
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Tech Serve Solutions is a global supplier and exporter of fine chemicals, handling the logistics for international shipment while adhering to all relevant regulations.
How can I request a sample or quote?
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Please contact our sales team via the website or direct inquiry to request a sample or a detailed quote for 4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate.
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