4-Methylumbelliferyl phosphate
4-Methylumbelliferyl phosphate (CAS: 3368-04-5) is a chemical compound with the formula C10H9O6P and a molecular weight of 256.15 g/mol. This substrate is primarily employed in biochemical assays for the detection and quantification of alkaline phosphatase activity. Its ability to yield a fluorescent product upon enzymatic cleavage makes it valuable in various research and diagnostic applications requiring sensitive detection methods.
- IUPAC
- 4-Methylumbelliferyl-phosphoric acid
- Synonyms
- 4-Methylumbelliferyl-phosphoric acid
Enzyme Activity Assays
4-Methylumbelliferyl phosphate serves as a crucial fluorogenic substrate for quantifying alkaline phosphatase activity in biological samples. The enzymatic hydrolysis releases 4-methylumbelliferone, which fluoresces upon excitation, enabling sensitive detection.
Diagnostic Kits
It is utilised in the development and manufacturing of diagnostic kits where alkaline phosphatase is an indicator enzyme. This includes applications in immunoassays and molecular diagnostics.
Biochemical Research
Researchers employ this compound in fundamental biochemical studies investigating enzyme kinetics, characterisation, and the development of enzyme inhibitors.
Fluorescence Microscopy
The fluorescent properties of the liberated 4-methylumbelliferone allow for its use in certain fluorescence-based imaging techniques in biological research.
| Molecular weight | 256.15 |
|---|---|
| Empirical formula | C10H9O6P |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|---|
| Description | phosphatase substrate |
| Fluorescence | λex 319 nm; λem 384 nm (pH 9.1)λex 360 nm; λem 449 nm (Reaction product) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Methylumbelliferyl phosphate used for?
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4-Methylumbelliferyl phosphate (CAS: 3368-04-5) is used as a fluorogenic substrate for the detection and quantification of alkaline phosphatase activity in biochemical assays and diagnostic kits.
What are the CAS number and formula for 4-Methylumbelliferyl phosphate?
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The CAS number is 3368-04-5 and the empirical formula is C10H9O6P.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Methylumbelliferyl phosphate at a PREMIUM quality level. We do not represent this product as USP, BP, EP, or other pharmacopoeial grades unless explicitly stated in the product specifications.
How should 4-Methylumbelliferyl phosphate be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Store at -20°C. Refer to the safety data sheet for comprehensive handling guidelines.
Is 4-Methylumbelliferyl phosphate a controlled substance?
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The provided data does not indicate that 4-Methylumbelliferyl phosphate is a controlled substance.
How are Tech Serve Solutions products packed and shipped?
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Tech Serve Solutions expertly packs and ships fine chemicals, pharmaceutical intermediates, and laboratory reagents globally, adhering to all necessary regulations for safe transport.
How can I request a quote or sample for 4-Methylumbelliferyl phosphate?
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To request a quote or sample, please contact our sales team through the website or directly via email, providing the product name and CAS number (3368-04-5).
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