4-Methylumbelliferyl phosphate disodium salt
4-Methylumbelliferyl phosphate disodium salt (CAS 22919-26-2, C10H7Na2O6P) is a biochemical reagent recognised for its utility as a chromogenic substrate. With a molecular weight of 300.11 g/mol, it is primarily employed in enzymatic assays, particularly for the detection and quantification of alkaline phosphatase activity. Its ability to yield a fluorescent product upon enzymatic cleavage makes it a valuable tool in biochemical research and diagnostics.
- IUPAC
- 4-MUP,Disodium 4-methylumbelliferyl phosphate
- Synonyms
- Disodium 4-methylumbelliferyl phosphate4-MUP4-Methylumbelliferyl phosphate, disodium salt
Enzyme Activity Assays
This compound serves as a sensitive fluorogenic substrate for the determination of alkaline phosphatase (AP) activity. Upon enzymatic hydrolysis by AP, it releases the fluorescent molecule 4-methylumbelliferone, allowing for quantitative measurements.
Biochemical Research
It is widely utilized in molecular biology and biochemistry laboratories to study enzyme kinetics, screen for enzyme inhibitors, and as a component in various diagnostic kits.
Fluorescence Detection
The product is ideal for assays requiring high sensitivity due to the fluorescent nature of its hydrolysis product, enabling detection at low enzyme concentrations.
ELISA and Protein Quantitation
This substrate can be incorporated into enzyme-linked immunosorbent assays (ELISA) and other protein quantitation methods where alkaline phosphatase is used as a reporter enzyme.
| Molecular weight | 300.11 |
|---|---|
| Empirical formula | C10H7Na2O6P |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|---|
| Description | phosphatase substrate |
| Fluorescence | λex 319 nm; λem 384 nm (pH 9.0)λex 360 nm; λem 449 nm (Reaction product) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Methylumbelliferyl phosphate disodium salt used for?
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4-Methylumbelliferyl phosphate disodium salt is used as a fluorogenic substrate for the detection and quantification of alkaline phosphatase (AP) activity in biochemical assays and research.
What is the CAS number and formula for 4-Methylumbelliferyl phosphate disodium salt?
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The CAS number is 22919-26-2 and the empirical formula is C10H7Na2O6P.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product at PREMIUM quality level. TSS does not represent this product as USP, BP, or EP grade; it is intended for research and laboratory use.
How should 4-Methylumbelliferyl phosphate disodium salt be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 or P1 respirator filter. Store at -20°C. This compound is classified under WGK Germany 3.
Is this product available for export and in what form is it supplied?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals. This product is supplied as a powder.
How can I request a sample or quote?
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Please contact our sales team via the website or by phone to request a sample or obtain a quotation for 4-Methylumbelliferyl phosphate disodium salt.
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