SQ-NHS
SQ-NHS (CAS: 154161-81-6; Formula: C34H32ClN3O6; MW: 614.09) is a squarain-carboxylate N-succinimidyl ester. This compound features a reactive N-hydroxysuccinimide group for efficient coupling with amino groups, making it valuable in biochemical applications. It exhibits high quantum yield, with stable absorbance, emission, and nanosecond lifetime in the physiological pH range (6-9). While insoluble in water, it is soluble in DMF.
- IUPAC
- Squarain-carboxylate N-succinimidyl ester
Fluorescent Labeling
SQ-NHS can be conjugated with proteins and other biomolecules via its reactive N-hydroxysuccinimide ester group, enabling their detection through fluorescence spectroscopy. Its favourable spectral properties and pH-independent fluorescence characteristics are advantageous for various biological assays.
Biochemical Research
Utilised in research settings for probing and labelling biological systems, SQ-NHS is suitable for fluorescence applications. Its lipophilic nature and membrane probe capabilities facilitate studies involving cellular structures and membranes.
Analytical Reagent
As an analytical reagent, SQ-NHS is employed in chromatography and spectroscopy due to its fluorescent properties. The compound's reliable absorbance and emission characteristics make it useful for quantitative analysis.
| Molecular weight | 614.09 |
|---|---|
| Empirical formula | C34H32ClN3O6 |
| Assay | >50% (coupling to amines) |
| Solubility | DMF: soluble |
| Storage temperature | 2-8°C |
| Application | SQ-NHS contains a reactive N-hydroxysuccinimide group, which allows for coupling with amino groups. When conjugated with proteins, SQ-NHS demonstrates a high quantum yield with an absorption maximum at 640 nm and a nanosecond lifetime. Additionally, in the physiological pH range (6-9), the absorbance, emission, and lifetime of SQ-NHS are pH-independent. It is water insoluble 1,2. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 600 nm; λem 645 nm in 0.1 M phosphate pH 7.0 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is SQ-NHS used for?
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SQ-NHS is used as a fluorescent labelling agent for biomolecules, in biochemical research as a lipophilic and membrane probe, and as an analytical reagent in spectroscopy and chromatography. Its primary function involves coupling with amino groups.
What are the CAS number and formula for SQ-NHS?
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The CAS number for SQ-NHS is 154161-81-6, and its empirical formula is C34H32ClN3O6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies SQ-NHS with an assay of >50% (coupling to amines). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade. It is suitable for fluorescence applications and research use.
What are the safety and handling considerations for SQ-NHS?
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SQ-NHS is classified with a WGK Germany of 3. Appropriate personal protective equipment, including eyeshields and gloves, should be used. A type N95 (US) or type P1 (EN143) respirator filter is recommended during handling.
How is SQ-NHS typically stored and shipped?
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SQ-NHS should be stored at 2-8°C. Tech Serve Solutions exports fine chemicals globally and ensures appropriate packaging and shipping procedures for sensitive reagents.
How can I request a quote or sample for SQ-NHS?
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To request a quote or a sample of SQ-NHS, please contact our sales department through the website or by phone.
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