Nitr 5/AM
Nitr 5/AM (CAS: 209161-73-9), with the empirical formula C43H47N3O23 and a molecular weight of 973.84 g/mol, is the acetoxymethyl ester of Nitr 5. This compound is designed for cellular loading, where it is subsequently cleaved by cytoplasmic esterases to release the active chelator. It serves as a valuable tool in biological research for intracellular calcium measurements and related studies.
- IUPAC
- Nitr 5 tetrakis(acetoxymethyl ester)
Intracellular Calcium Imaging
Nitr 5/AM is employed as a fluorescent indicator for measuring intracellular calcium concentrations. Its ester form allows for cell membrane penetration, and subsequent enzymatic cleavage releases the active indicator within the cytoplasm.
Flow Cytometry
This compound is suitable for use in flow cytometry applications, enabling the quantitative analysis of intracellular calcium levels in single cells.
Fluorescence Microscopy
Nitr 5/AM is utilised in fluorescence microscopy for visualising and quantifying dynamic changes in intracellular calcium in cellular and tissue samples.
| Molecular weight | 973.84 |
|---|---|
| Empirical formula | C43H47N3O23 |
| Assay | ≥90% (HPLC) |
| Storage temperature | −20°C |
| Application | Acetoxymethyl ester of Nitr 5. For loading the chelator into the cell where it is cleaved by cytoplasmic esterases. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Shipped In | wet ice |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nitr 5/AM used for?
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Nitr 5/AM is used as an acetoxymethyl ester for loading the chelator Nitr 5 into cells, where it is cleaved by cytoplasmic esterases for intracellular calcium measurements and related fluorescence-based assays.
What are the CAS number and formula for Nitr 5/AM?
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The CAS number for Nitr 5/AM is 209161-73-9, and its empirical formula is C43H47N3O23.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Nitr 5/AM with an assay of ≥90% (HPLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety and handling recommendations for Nitr 5/AM?
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Handling Nitr 5/AM requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The compound is classified with WGK Germany of 3.
How is Nitr 5/AM shipped and stored?
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Nitr 5/AM is shipped in wet ice and should be stored at -20°C to maintain its stability and integrity.
How can I request a sample or quote for Nitr 5/AM?
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To request a sample or obtain a quote for Nitr 5/AM, please contact our sales team via the website or direct inquiry.
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