Nile Red
Nile Red (CAS: 7385-67-3, C20H18N2O2) is a fluorescent stain and lipophilic probe with a molecular weight of 318.37 g/mol. It is primarily employed in biological and chemical research for the selective staining of lipid-rich cellular components, including lipid droplets and membranes. Its strong fluorescence at λmax 553 nm makes it invaluable for microscopic imaging and analytical studies requiring the visualisation of lipophilic structures in various biological systems.
- IUPAC
- Nile Blue A Oxazone

Lipid Droplet Staining
Nile Red is widely used to identify and quantify lipid droplets within cells and tissues due to its selective affinity for neutral lipids. Its fluorescence allows for direct visualisation under a microscope, aiding in the study of lipid metabolism and storage.
Membrane Staining
The lipophilic nature of Nile Red enables it to intercalate into cell membranes, making it useful for visualising membrane structure and dynamics. This application is crucial in cell biology research for understanding membrane integrity and function.
Fluorescent Probe in Research
As a versatile fluorescent probe, Nile Red is employed in various analytical techniques. Its distinct spectral properties are leveraged in assays for detecting and quantifying lipophilic substances in diverse chemical and biological matrices.
| Molecular weight | 318.37 |
|---|---|
| Empirical formula | C20H18N2O2 |
| Solubility | methanol: soluble1 mg/mL |
| Application | Do you have application information on this product that you would like to share? |
| Melting point | 203-205 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Absorption | λmax 553 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nile Red used for?
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Nile Red is used as a fluorescent stain and lipophilic probe for identifying and visualising lipid-rich structures such as lipid droplets and cell membranes in biological research.
What is the CAS number and formula for Nile Red?
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The CAS number for Nile Red is 7385-67-3, and its molecular formula is C20H18N2O2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Nile Red as a BioReagent grade. TSS does not represent this product as USP, BP, EP or other pharmacopoeial grades.
What are the safety precautions when handling Nile Red?
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When handling Nile Red, it is essential to wear eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Nile Red is classified under WGK Germany 3, indicating significant environmental hazard.
How is Nile Red typically packed and shipped?
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Nile Red is carefully packed to ensure stability during transit and is exported globally by Tech Serve Solutions, adhering to all relevant shipping regulations for chemical substances.
How can I request a sample or quote for Nile Red?
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To request a sample or a quote for Nile Red, please contact our sales team through the website or via the provided contact details.
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