HEPES sodium salt

CAS 75277-39-3C8H17N2NaO4SBiochemicals & Reagents

HEPES sodium salt (CAS: 75277-39-3), a derivative of HEPES, is a zwitterionic buffering agent with the empirical formula C8H17N2NaO4S and a molecular weight of 260.29 g/mol. It is highly soluble in water and maintains a stable pH in the physiological range, making it an excellent choice for biological research and cell culture applications. This PharmaGrade buffer is rigorously tested to ensure its suitability for sensitive biological systems.

IUPAC: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt,N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt,N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

Email us

HEPES sodium salt (C8H17N2NaO4S) — chemical structure, CAS 75277-39-3; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsBiological BuffersGood’s BuffersPharmaGrade Buffers

01 /Applications

Cell Culture Media

HEPES sodium salt is utilized as a buffering agent in cell culture media to maintain stable physiological pH, crucial for cell viability and growth. Its high buffering capacity prevents drastic pH fluctuations caused by cellular metabolic activity.

Biochemical Assays

This compound serves as a reliable buffer in various biochemical and enzymatic assays where consistent pH is critical for optimal reaction rates and stability of biomolecules. It is tested for the absence of cytotoxic, DNase, nickase, RNase, and protease activities.

Electrophoresis and Chromatography

In electrophoresis and chromatography, HEPES sodium salt can be employed to maintain buffer pH, ensuring consistent separation and analysis of biological samples. Its high purity minimizes interference with analytical results.

Pharmaceutical Research

As a PharmaGrade buffer, it is suitable for use in pharmaceutical research and development, including studies involving protein purification and formulation, where stringent quality standards are required.

02 /Properties

Molecular weight	260.29
Empirical formula	C8H17N2NaO4S
Assay	≥99.5%
Form	powder
Solubility	H2O: soluble1 M, clear, colorless
pH	10.0-12.0 (1 M in H2O)
Impurities tested	endotoxin and total aerobic microbial count, tested
Suitability	suitable for cell culture
Biological source	from synthetic

03 /Safety & handling

Water hazard class (WGK, DE)	3
Cation traces	Fe: ≤5 ppmheavy metals (as Pb): ≤5 ppm
Anion traces	chloride (Cl-): ≤0.05%sulfate (SO42-): ≤0.05%

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

75277-39-3

05 /Additional specifications

Quality Level	ELITE
Product Line	PharmaGrade
Pka 25 &#X00b0;C	7.5
Foreign Activity	Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
Absorption	A1M/260, H2O ≤0.4A1M/280, H2O ≤0.2
Sterility	sterile; 0.2 μm filtered
Useful Ph Range	6.8 - 8.2

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is HEPES sodium salt used for?

HEPES sodium salt is primarily used as a buffering agent in cell culture, biochemical assays, electrophoresis, and pharmaceutical research to maintain stable physiological pH. It is tested for suitability in cell culture and absence of common biological contaminants.

What are the CAS number and formula for HEPES sodium salt?

The CAS number for HEPES sodium salt is 75277-39-3, and its empirical formula is C8H17N2NaO4S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies HEPES sodium salt at an assay of ≥99.5% purity, designated as PharmaGrade. It is tested for sterility and common biological impurities.

What are the safety and handling precautions for HEPES sodium salt?

HEPES sodium salt is classified with WGK Germany 2, indicating it is hazardous to water. Handle with appropriate personal protective equipment in a well-ventilated area to avoid dust inhalation and skin contact. Refer to the Safety Data Sheet for detailed handling information.

Is HEPES sodium salt a controlled substance?

No, HEPES sodium salt is not listed as a controlled or scheduled substance.

How is HEPES sodium salt packed and shipped?

HEPES sodium salt is available as a sterile powder and is exported globally by Tech Serve Solutions in compliance with all relevant shipping regulations.