HEPES potassium salt
HEPES potassium salt (CAS: 82207-62-3, Formula: C8H17KN2O4S) is a zwitterionic organic chemical compound with a molecular weight of 276.39 g/mol. It functions as a biological buffer, offering excellent buffering capacity within the physiological pH range. This compound is widely utilised in biochemical research and cell culture media to maintain stable pH conditions, crucial for numerous biological assays and processes.
- IUPAC
- 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt,N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) potassium salt
Biological Buffering
HEPES potassium salt is employed as a buffering agent in biological and biochemical research. Its pKa of 7.5 allows it to effectively maintain pH between 6.8 and 8.2, which is vital for enzyme activity and cellular function in vitro.
Cell Culture Media
It is a common component in cell culture media formulations. By stabilising the pH, it supports cell viability and growth, preventing damage from pH fluctuations that can occur due to cellular metabolism.
Biochemical Assays
This buffer is used in various biochemical assays where precise pH control is critical for the reliability and accuracy of experimental results, such as protein purification and enzyme kinetics studies.
| Molecular weight | 276.39 |
|---|---|
| Empirical formula | C8H17KN2O4S |
| Assay | ≥99.5% (titration) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Pka  | 7.5 |
|---|---|
| Useful Ph Range | 6.8 - 8.2 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is HEPES potassium salt used for?
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HEPES potassium salt is primarily used as a biological buffer in research settings, particularly in cell culture media and biochemical assays, to maintain stable pH conditions between 6.8 and 8.2.
What are the CAS number and formula for HEPES potassium salt?
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The CAS number for HEPES potassium salt is 82207-62-3, and its empirical formula is C8H17KN2O4S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies HEPES potassium salt with an assay of ≥99.5% (titration). We do not represent this product as USP, BP, or EP grade.
What are the safety and handling precautions for HEPES potassium salt?
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Handling HEPES potassium salt requires the use of safety equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne exposure is possible. It is classified under WGK Germany 3.
How is HEPES potassium salt typically packed and exported?
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HEPES potassium salt is carefully packed to ensure stability during transit and is available for global export by Tech Serve Solutions.
How can I request a quote or sample for HEPES potassium salt?
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To request a quote or a sample of HEPES potassium salt, please contact our sales team through the TSS website or your designated account manager.
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