HEPES

CAS 7365-45-9C8H18N2O4SBiochemicals & Reagents

HEPES (CAS: 7365-45-9; Formula: C8H18N2O4S; MW: 238.30 g/mol) is a zwitterionic organic chemical buffering agent widely utilized in biochemical research. It is particularly favoured for its minimal interaction with biological macromolecules and its pKa of 7.5 at 25°C, maintaining stable pH within the physiologically relevant range of 6.8 to 8.2. HEPES is suitable for applications such as mouse embryo cell culture and is a key component in diagnostic assay manufacturing.

IUPAC: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid,N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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HEPES (C8H18N2O4S) — chemical structure, CAS 7365-45-9; Life Science, fine chemical supplied by Tech Serve Solutions

Apoptosis and Cell CycleBiochemicals and ReagentsBiological BuffersBuffersBuffers A to ZCell BiologyGood’s Buffers

01 /Applications

Biological Buffering

HEPES is an effective zwitterionic buffer with a pKa of 7.5, making it suitable for maintaining a stable pH between 6.8 and 8.2 in biological systems. It is widely employed in cell culture media and biochemical assays due to its low metal ion binding and minimal interaction with biological molecules.

Cell Culture

This compound is recognised for its suitability in mouse embryo cell culture, contributing to stable culture conditions. Its buffering capacity helps to prevent detrimental pH fluctuations that can affect cell viability and growth in sensitive biological applications.

Diagnostic Assays

HEPES is a critical component in the manufacturing of diagnostic assays, where precise pH control is essential for assay performance and reliability. Its high purity and consistent buffering capacity ensure reproducible results.

Biochemical Research

As a biochemical reagent, HEPES is valuable in various research settings, including studies on apoptosis, cell cycle, and cell signalling. Its properties minimise interference with experimental outcomes, supporting accurate scientific investigations.

02 /Properties

Molecular weight	238.30
Empirical formula	C8H18N2O4S
Assay	≥99.5% (titration)
Density	1.07 g/mL at 20 °C
Solubility	H2O: soluble1 M at 20 °C, clear, colorless
pH	5.0-6.5 (25 °C, 1 M in H2O)
Concentration	1 M in H2O238 g/L HEPES in H2O
Loss on drying	≤0.5% loss on drying, 110 °C
Impurities tested	DNases, none detectedRNases, none detectedinsoluble matter, passes filter testphosphatases, none detectedproteases, none detected
Suitability	suitable for mouse embryo cell culture

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	1
Anion traces	chloride (Cl-): ≤50 mg/kgsulfate (SO42-): ≤50 mg/kg
Ignition residue	≤0.1% (as SO4)

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

7365-45-9

MDL number

MFCD00006158

PubChem substance

24895572

Beilstein registry

883043

05 /Additional specifications

Quality Level	ELITE
Pka 25 &#X00b0;C	7.5
Market Segment	Diagnostic Assay Manufacturing
Uv Absorption	λ: 260 nm Amax: 0.045λ: 280 nm Amax: 0.035
Absorption	A33%/290, H2O ≤0.05
Sterility	0.2 μm filtered
Useful Ph Range	6.8 - 8.2

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is HEPES used for?

HEPES is primarily used as a biological buffer to maintain stable pH in the range of 6.8-8.2, particularly in cell culture applications such as mouse embryo cell culture, and in the manufacturing of diagnostic assays.

What is the CAS number and chemical formula for HEPES?

The CAS number for HEPES is 7365-45-9, and its chemical formula is C8H18N2O4S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies HEPES at an ELITE quality level with an assay of ≥99.5% (titration). TSS does not represent this product as USP, BP, or EP grade.

How should HEPES be handled safely?

When handling HEPES, it is recommended to use protective equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if necessary. Refer to the safety data sheet for comprehensive handling information.

Is HEPES a controlled substance?

The provided data does not indicate that HEPES is a controlled substance.

How is HEPES packed and exported?

Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. Specific packing and export details can be provided upon request.

How can I request a sample or quote for HEPES?

To request a sample or a quote for HEPES, please contact the Tech Serve Solutions sales team directly through our website or designated contact channels.