HEPES hemisodium salt
HEPES hemisodium salt (CAS: 103404-87-1) is a zwitterionic organic chemical buffer, a derivative of HEPES. With the molecular formula C8H17.5N2O4S · 0.5Na and a molecular weight of 249.30, it offers excellent buffering capacity in the physiological pH range. This compound is widely utilised in biological research and diagnostic assay manufacturing due to its stability and minimal interaction with biological molecules.
- IUPAC
- 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt,N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) hemisodium salt

Biological Buffering
HEPES hemisodium salt is an effective buffer for cell culture media, enzyme assays, and other biochemical applications, maintaining pH stability between 6.8 and 8.2.
Diagnostic Assay Manufacturing
Its reliable buffering properties make it a key component in the production of various diagnostic assays and kits where precise pH control is critical for performance.
Biotechnology Research
This buffer is employed in numerous biotechnological processes, including protein purification and studies involving nucleic acids, ensuring optimal conditions for biomolecular interactions.
| Molecular weight | 249.30 |
|---|---|
| Empirical formula | C8H17.5N2O4S · 0.5Na |
| Assay | ≥99% (titration) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Pka  | 7.5 |
|---|---|
| Market Segment | Diagnostic Assay Manufacturing |
| Useful Ph Range | 6.8 - 8.2 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is HEPES hemisodium salt primarily used for?
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HEPES hemisodium salt is primarily used as a biological buffer in cell culture, biochemical assays, and diagnostic assay manufacturing due to its effective pH range and stability.
What is the CAS number and formula for HEPES hemisodium salt?
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The CAS number for HEPES hemisodium salt is 103404-87-1, and its formula is C8H17.5N2O4S · 0.5Na.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies HEPES hemisodium salt with an assay of ≥99% (titration). We do not represent this product as USP, BP, or EP grade.
How should HEPES hemisodium salt be handled safely?
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Handle HEPES hemisodium salt with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The substance has a WGK Germany rating of 3, indicating significant hazard.
How is HEPES hemisodium salt typically shipped or exported?
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HEPES hemisodium salt is carefully packaged and exported by TSS, adhering to international shipping regulations for chemical substances to ensure safe and compliant delivery worldwide.
How can I request a sample or quote for HEPES hemisodium salt?
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To request a sample or a quote for HEPES hemisodium salt, please contact our sales team through the TSS website or by direct inquiry, providing your specific requirements.
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