G 418 disulfate salt

CAS 108321-42-2C20H40N4O10 · 2H2SO4Biochemicals & Reagents

G 418 disulfate salt (CAS: 108321-42-2), with the empirical formula C20H40N4O10 · 2H2SO4 and a molecular weight of 692.71, is an aminoglycoside antibiotic. It is primarily utilised for the selection and maintenance of prokaryotic and eukaryotic cells that have been transfected with constructs incorporating a neomycin resistance gene. This sterile-filtered powder, suitable for plant cell culture, requires specific handling due to its hazardous properties.

IUPAC: Antibiotic G418

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G 418 disulfate salt (C20H40N4O10 · 2H2SO4) — chemical structure, CAS 108321-42-2; Life Science, fine chemical supplied by Tech Serve Solutions

AminoglycosidesAntibacterialAntibiotic powdersAntibioticsAntibiotics A to ZAntibiotics G-MAntibiotics by ApplicationBiochemicals and ReagentsCell CultureChemical Structure Class

01 /Applications

Cell Line Selection

G 418 disulfate salt serves as an effective antibiotic for selecting cells modified to express neomycin resistance. It aids in maintaining the genetic integrity of transfected cell lines in research settings.

Eukaryotic Cell Culture

This compound is applied in the culture of eukaryotic cells to ensure the stable expression of desired genes, particularly when a neomycin resistance cassette is employed for selection. Optimal concentrations vary by cell type, with animal cells typically requiring 300-500 μg/mL.

Prokaryotic Cell Culture

It is also used for the selection and maintenance of prokaryotic cells harbouring resistance genes. For bacteria, the effective selection concentration is generally 5 μg/mL or less.

Genetic Marker Application

G 418 disulfate salt functions as a crucial tool in genetic engineering and molecular biology for marker-assisted selection, ensuring that only cells successfully incorporating specific genetic material are maintained.

02 /Properties

Molecular weight	692.71
Empirical formula	C20H40N4O10 · 2H2SO4
Form	powder
Solubility	H2O: soluble50 mg/mL
Concentration	50 mg/mL in H2O
Storage temperature	2-8°C
Impurities tested	endotoxin, tested
Application	G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.
Suitability	plant cell culture tested

03 /Safety & handling

GHS hazard pictogram: Health hazard (GHS08) — Health hazard

Danger

Hazard statements

H317May cause an allergic skin reaction
H334May cause allergy or asthma symptoms if inhaled

Precautionary statements

P261Avoid breathing dust, fume, gas or vapours
P280Wear protective gloves, clothing and eye/face protection
P342

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xn
Risk statements (R)	42/43
Safety statements (S)	22-36/37-45

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

108321-42-2

MDL number

MFCD00058314

PubChem substance

24890581

05 /Additional specifications

Product Line	BioReagent
Sterility	sterile-filtered
Potency	≥450 μg per mg (dried basis)

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is G 418 disulfate salt used for?

G 418 disulfate salt is an aminoglycoside antibiotic used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with a neomycin resistance gene.

What are the CAS number and formula for G 418 disulfate salt?

The CAS number for G 418 disulfate salt is 108321-42-2, and its empirical formula is C20H40N4O10 · 2H2SO4.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies G 418 disulfate salt as a powder with a potency of ≥450 μg per mg (dried basis). It is tested for endotoxins and is suitable for plant cell culture. TSS does not represent this product as USP, BP, or EP grade.

How should G 418 disulfate salt be handled safely?

G 418 disulfate salt is classified as a danger. It may cause an allergic skin reaction (H317) and may cause allergy or asthma symptoms or breathing difficulties if inhaled (H334). Always use appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator. Handle in a well-ventilated area and avoid generating dust. Follow all recommended safety precautions and emergency procedures.

Is G 418 disulfate salt available for export?

Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals and laboratory reagents, including G 418 disulfate salt.

How can I request a quote or sample for G 418 disulfate salt?

To request a quote or sample for G 418 disulfate salt, please contact our sales department via the website or by phone. Our team will be pleased to assist you.