Auramine O
Auramine O (CAS 2465-27-2) is a diarylmethane dye with the chemical formula C17H21N3 · HCl and a molecular weight of 303.83 g/mol. As a fluorescent yellow dye, it is widely recognised for its biological staining properties. Certified by the Biological Stain Commission, it is primarily employed in microscopy and histology for the detection of acid-fast bacilli and in various fluorescence-based research applications within cell biology.
- IUPAC
- 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride,Basic Yellow 2,Pyoctaninum aureum
- Synonyms
- Basic Yellow 24,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloridePyoctaninum aureum
Histology and Cytology Staining
Auramine O is a certified stain used for the fluorescent microscopy of acid-fast bacilli, such as Mycobacteria. Its strong fluorescence allows for sensitive detection in clinical and research settings.
Biological Research
As a fluorescent label and probe, Auramine O is utilised in cell biology for various imaging techniques. It aids in visualising cellular structures and processes under fluorescence microscopy.
Diagnostic Staining
This dye serves as a critical reagent in diagnostic laboratories for identifying specific microorganisms, particularly in tuberculosis testing, due to its high affinity for certain cellular components.
| Molecular weight | 303.83 |
|---|---|
| Empirical formula | C17H21N3 · HCl |
| Grade | certified by the Biological Stain Commission |
| Composition | Dye content, ≥80% |
| Melting point | >250 °C (dec.)(lit.) |
Hazard statements
- H302Harmful if swallowed
- H311Toxic in contact with skin
- H351Suspected of causing cancer
Precautionary statements
- P280Wear protective gloves, clothing and eye/face protection
- P312Call a POISON CENTER if you feel unwell
| Transport (UN / ADR) | UN 2811 6.1 / PGIII |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 22-24-40 |
| Safety statements (S) | 36/37-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 41000 |
|---|---|
| Absorption | λmax 370 nmλmax 434 nm (2nd) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Auramine O used for?
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Auramine O is primarily used as a fluorescent stain in histology and cytology for the detection of acid-fast bacilli, such as Mycobacteria, in microscopy. It also finds applications as a fluorescent probe in cell biology research.
What is the CAS number and chemical formula for Auramine O?
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The CAS number for Auramine O is 2465-27-2, and its chemical formula is C17H21N3 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Auramine O certified by the Biological Stain Commission with a dye content of ≥80%. We do not represent this product as meeting USP, BP, or EP pharmacopoeial standards.
What are the safety and handling considerations for Auramine O?
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Auramine O is classified as dangerous. It is harmful if swallowed (H302), toxic in contact with skin (H311), and suspected of causing cancer (H351). Handling requires protective clothing, gloves, and eye protection, and should be performed in a well-ventilated area. Consult the Safety Data Sheet for full details.
How is Auramine O packed, shipped, and exported?
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Auramine O is packed according to relevant regulations for hazardous materials, including UN 2811 classification. Tech Serve Solutions has extensive experience in global shipping and exporting fine chemicals.
How can I request a sample or quote for Auramine O?
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To request a sample or a quote for Auramine O, please contact our sales team through the website or by phone, providing your specific requirements.
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