Atto 590 NHS ester
Atto 590 NHS ester (CAS: 670269-33-7; Formula: C41H42ClN3O11; MW: 788.24 g/mol) is a highly fluorescent labelling reagent suitable for amine coupling. It exhibits strong absorption at 593 nm and emission around 620 nm, with a high molar absorptivity of 120,000 L/M·cm and a quantum yield of 0.93. This makes it an excellent choice for various biological and chemical detection applications requiring sensitive fluorescent probes.

Fluorescent Labelling of Biomolecules
Atto 590 NHS ester can be covalently attached to primary amines in proteins, peptides, and other biomolecules. This facilitates their detection and tracking in biological systems using fluorescence microscopy or flow cytometry.
Nucleic Acid Staining
The fluorescent properties of Atto 590 NHS ester make it suitable for staining nucleic acids. It can be employed in various assays for visualizing DNA and RNA in research and diagnostic applications.
Detection Assays
With its high quantum yield and photostability, this NHS ester is valuable for developing sensitive detection assays. It can be used in immunoassays or other proximity-based detection systems where a fluorescent signal is required.
| Molecular weight | 788.24 |
|---|---|
| Empirical formula | C41H42ClN3O11 |
| Assay | ≥60% (coupling to amines) |
| Storage temperature | −20°C |
| Application | Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, Tfl 4.0 ns (unpublished data) |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 590 nm; λem ~620 nm in 0.1 M phosphate pH 7.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Atto 590 NHS ester used for?
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Atto 590 NHS ester is used for fluorescent labelling of biomolecules, nucleic acid staining, and in various sensitive detection assays.
What are the CAS number and formula for Atto 590 NHS ester?
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The CAS number is 670269-33-7, and the empirical formula (Hill notation) is C41H42ClN3O11.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Atto 590 NHS ester with an assay of ≥60% (coupling to amines). This product is suitable for research applications. Tech Serve Solutions does not represent this product as USP, BP, EP, or other pharmacopoeial grades.
How should Atto 590 NHS ester be handled safely?
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When handling Atto 590 NHS ester, it is essential to wear appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used. The substance has a WGK Germany classification of 3.
How is Atto 590 NHS ester packaged and shipped?
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Atto 590 NHS ester is typically stored at -20°C. Tech Serve Solutions professionally packages and exports fine chemicals globally, adhering to all necessary regulations for safe transportation.
How can I request a sample or quote for Atto 590 NHS ester?
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To request a sample or a quote for Atto 590 NHS ester, please contact our sales team through the website or by phone. We are happy to assist with your research needs.
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