Ala-Ala-Phe p-nitroanilide
Ala-Ala-Phe p-nitroanilide (CAS 61043-41-2) is a vital biochemical reagent with the molecular formula C21H25N5O5 and a molecular weight of 427.45 g/mol. This compound serves as a specific substrate in enzymatic assays. Its primary utility lies in its application for the characterisation and study of enzyme activity, particularly for chymotrypsin and tripeptidyl peptidase I, contributing to biochemical research and diagnostic development.

Enzymatic Assays
Ala-Ala-Phe p-nitroanilide acts as a chromogenic substrate for the quantitative analysis of enzyme activity. Upon enzymatic cleavage, it releases p-nitroaniline, which can be measured spectrophotometrically.
Chymotrypsin Characterisation
This compound is a well-established substrate for chymotrypsin, enabling researchers to study its kinetics and inhibition profiles.
Tripeptidyl Peptidase I Studies
It is also employed as a substrate for tripeptidyl peptidase I, facilitating research into the function and regulation of this enzyme.
Biochemical Research Reagent
As a specialised reagent, it supports fundamental biochemical research, particularly in the fields of enzymology and protein science.
| Molecular weight | 427.45 |
|---|---|
| Empirical formula | C21H25N5O5 |
| Storage temperature | −20°C |
| Application | Substrate for chymotrypsin and tripeptidyl peptidase I. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Ala-Ala-Phe p-nitroanilide used for?
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Ala-Ala-Phe p-nitroanilide is used as a chromogenic substrate for studying the activity of enzymes such as chymotrypsin and tripeptidyl peptidase I in biochemical research.
What are the CAS number and formula for Ala-Ala-Phe p-nitroanilide?
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The CAS number for Ala-Ala-Phe p-nitroanilide is 61043-41-2, and its molecular formula is C21H25N5O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Ala-Ala-Phe p-nitroanilide as a research-grade chemical. The specific assay and purity are detailed in the product's specification sheet; TSS does not represent this product as USP, BP, or EP grade.
How should Ala-Ala-Phe p-nitroanilide be handled and stored?
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Handle Ala-Ala-Phe p-nitroanilide with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at -20°C.
Is Ala-Ala-Phe p-nitroanilide a hazardous substance?
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According to available safety data, Ala-Ala-Phe p-nitroanilide has a WGK Germany classification of 3, indicating it is hazardous to water. Appropriate protective measures must be taken during handling.
How can I request a quote or sample for Ala-Ala-Phe p-nitroanilide?
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To request a quote or a sample of Ala-Ala-Phe p-nitroanilide, please contact our sales team through the website or by phone. Our team will assist you with your inquiry and provide necessary documentation.
Does Tech Serve Solutions export Ala-Ala-Phe p-nitroanilide?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including Ala-Ala-Phe p-nitroanilide, serving customers worldwide.
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