Ala-Ala-Phe-7-amido-4-methylcoumarin
Ala-Ala-Phe-7-amido-4-methylcoumarin (CAS: 62037-41-6) is a synthetic peptide derivative with the molecular formula C25H28N4O5 and a molecular weight of 464.51 g/mol. This compound serves as a valuable fluorogenic substrate, primarily utilized in biochemical research for the detection and quantification of enzyme activity. Its specific application involves monitoring proteases that cleave the Ala-Ala-Phe sequence.
Fluorogenic Enzyme Substrate
Ala-Ala-Phe-7-amido-4-methylcoumarin is designed as a fluorogenic substrate for enzymes that hydrolyse peptide bonds. Upon cleavage by specific proteases, it releases a fluorescent 7-amino-4-methylcoumarin moiety, enabling sensitive detection and quantification of enzyme activity in biochemical assays.
Protease Activity Monitoring
This compound is particularly useful for studying proteases that recognise and cleave the Ala-Ala-Phe sequence. It allows researchers to investigate enzyme kinetics, inhibition, and specificity in various biological contexts, contributing to a deeper understanding of cellular processes.
Cell Biology Research
As a reagent in cell biology, Ala-Ala-Phe-7-amido-4-methylcoumarin can be employed to probe protease function within cellular environments. Its application aids in understanding cell signalling pathways and other cellular mechanisms regulated by proteolytic activity.
| Molecular weight | 464.51 |
|---|---|
| Empirical formula | C25H28N4O5 |
| Storage temperature | 2-8°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Ala-Ala-Phe-7-amido-4-methylcoumarin used for?
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Ala-Ala-Phe-7-amido-4-methylcoumarin is primarily used as a fluorogenic substrate for the detection and quantification of protease activity, particularly those that cleave the Ala-Ala-Phe sequence. It is valuable in biochemical research and cell biology studies.
What is the CAS number and molecular formula?
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The CAS number for Ala-Ala-Phe-7-amido-4-methylcoumarin is 62037-41-6, and its molecular formula is C25H28N4O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Ala-Ala-Phe-7-amido-4-methylcoumarin as a research-grade chemical. TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade, and specific purity should be confirmed via a Certificate of Analysis.
What are the safety and handling requirements?
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When handling Ala-Ala-Phe-7-amido-4-methylcoumarin, it is essential to use personal protective equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is also recommended. The compound has a WGK Germany rating of 3, indicating a high hazard.
How is this chemical shipped and exported?
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Tech Serve Solutions is an experienced global supplier and exporter of fine chemicals, including Ala-Ala-Phe-7-amido-4-methylcoumarin. We ensure appropriate packaging and documentation for international shipment, adhering to all relevant regulations.
How can I request a sample or quote?
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To request a sample or a quotation for Ala-Ala-Phe-7-amido-4-methylcoumarin, please contact our sales department through the contact details provided on our website.
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