5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside (CAS: 15548-60-4) is a chromogenic substrate with a molecular weight of 408.63 g/mol. It is primarily utilised in biochemical research and molecular biology applications for the detection of enzyme activity. This compound is particularly valuable in microbiology and genetics for identifying and differentiating microorganisms or gene expression based on the presence of specific glycosidase enzymes.
- IUPAC
- X-Glc,X-glucoside
- Synonyms
- X-GlcX-glucoside

Chromogenic Enzyme Substrate
Used as a substrate for detecting beta-glucuronidase activity. Upon enzymatic cleavage, it releases a coloured precipitate, allowing for visual identification of enzyme presence and activity in various biological samples.
Molecular Biology and Genetics
Applied in gene expression studies and microbial identification. The colour change produced by the enzyme's action can be used as a reporter system to confirm gene cloning or to differentiate bacterial colonies possessing specific enzymatic functions.
Microbiology and Diagnostics
Facilitates the detection and differentiation of microorganisms in diagnostic assays. Its specificity for certain enzymes aids in identifying particular bacterial strains or assessing their metabolic profiles.
| Molecular weight | 408.63 |
|---|---|
| Empirical formula | C14H15BrClNO6 |
| Assay | ≥97% |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside used for?
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5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside is primarily used as a chromogenic substrate for detecting beta-glucuronidase activity in biochemical research, molecular biology, microbiology, and diagnostic applications. It produces a coloured precipitate upon enzymatic cleavage.
What are the CAS number and formula for this compound?
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The CAS number is 15548-60-4. The empirical formula is C14H15BrClNO6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside with an assay of ≥97%. TSS does not represent this product as USP, BP, or EP grade.
How should 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Store at -20°C to maintain stability.
Is 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside a controlled substance?
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The provided data does not indicate that 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside is a controlled substance.
How is this product packed, shipped, and exported?
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Tech Serve Solutions specialises in the global supply and export of fine chemicals. Specific packing and shipping details for 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside are available upon request.
How can I request a sample or a quote for 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside?
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To request a sample or a quote, please contact our sales team through the TSS website or via direct inquiry, providing the product name and CAS number.
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