N,N′-Methylenebisacrylamide
N,N′-Methylenebisacrylamide (CAS: 110-26-9), with the molecular formula (CH2=CHCONH)2CH2 and a molecular weight of 154.17 g/mol, is a key cross-linking agent. It is essential for the preparation of polyacrylamide gels, widely utilised in protein and nucleic acid electrophoresis. Supplied by Tech Serve Solutions, this reagent is crucial for molecular biology and life science research applications demanding high-resolution separation techniques.
- IUPAC
- BIS
- Synonyms
- (CH2=CHCONH)2CH2BIS
Polyacrylamide Gel Preparation
N,N′-Methylenebisacrylamide is used as a cross-linker when preparing polyacrylamide gels. It co-polymerizes with acrylamide monomers to form a stable gel matrix crucial for electrophoresis.
Protein Electrophoresis
The resulting polyacrylamide gels are employed for the separation of proteins based on their size and charge. This technique is fundamental in biochemical research and diagnostics.
Nucleic Acid Electrophoresis
This compound is integral to creating gels for separating DNA and RNA molecules. It enables precise analysis of nucleic acids in molecular biology laboratories.
Molecular Biology Research
As a core bioreagent, it supports various molecular biology applications, including Western blotting and nucleic acid analysis, contributing to essential research workflows.
| Molecular weight | 154.17 |
|---|---|
| Linear formula | (CH2=CHCONH)2CH2 |
| Assay | ≥99.5% |
| Grade | for molecular biology |
| Concentration | 2% in H2O |
| Storage temperature | 2-8°C |
| Impurities tested | ≤0.05% Free carboxylic acid (T)≤50 mg/kg Total sulfur (as SO4) (ICP) |
| Application | To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis. |
| Suitability | in accordance for electrophoresis test |
Hazard statements
- H302Harmful if swallowed
- H332Harmful if inhaled
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 2 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/22 |
| Safety statements (S) | 22 |
| Cation traces | Ca: ≤10 ppmCd: ≤5 ppmCo: ≤5 ppmCr: ≤5 ppmCu: ≤5 ppmFe: ≤5 ppmK: ≤50 ppmMg: ≤5 ppmMn: ≤5 ppmNa: ≤50 ppmNi: ≤5 ppmPb: ≤5 ppmZn: ≤5 ppm |
| Anion traces | chloride (Cl-): ≤50 mg/kg |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Foreign Activity | DNase, RNase, NICKase and protease, none detected |
|---|---|
| Absorption | A1%/290 ≤0.2 |
| Conductivity | ≤10 μmho (2% solution) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N,N′-Methylenebisacrylamide used for?
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N,N′-Methylenebisacrylamide is primarily used as a cross-linking agent in the preparation of polyacrylamide gels for protein and nucleic acid electrophoresis.
What is the CAS number and chemical formula for N,N′-Methylenebisacrylamide?
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The CAS number is 110-26-9, and its linear chemical formula is (CH2=CHCONH)2CH2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies N,N′-Methylenebisacrylamide with an assay of ≥99.5% and a grade suitable for molecular biology. We do not represent this product as USP, BP, or EP grade.
What are the main safety hazards associated with N,N′-Methylenebisacrylamide?
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N,N′-Methylenebisacrylamide is classified with GHS07 symbol and carries a Warning signal word. It is harmful if swallowed (H302) and harmful if inhaled (H332). Appropriate personal protective equipment, including a dust mask (type N95), eyeshields, and gloves, should be used during handling.
How is N,N′-Methylenebisacrylamide packed, shipped, and exported?
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N,N′-Methylenebisacrylamide is carefully packaged to maintain its integrity during transport. Tech Serve Solutions, established in 1998, is a specialist global supplier and exporter of fine chemicals, ensuring reliable international delivery.
How can I request a sample or quote for N,N′-Methylenebisacrylamide?
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To request a sample or a quotation for N,N′-Methylenebisacrylamide, please contact our sales team through the TSS website or direct inquiry channels.
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