N,N′-Bis(acryloyl)cystamine
N,N′-Bis(acryloyl)cystamine (CAS: 60984-57-8) is a BioReagent grade chemical with a molecular weight of 260.38. This reversible cross-linker is specifically utilised in the preparation of polyacrylamide gels for protein electrophoresis. Its unique disulfide linkage allows for the facile isolation of macromolecules separated on gels by subsequent reduction.
- IUPAC
- 2,2′-(Bisacrylamino)diethyl disulfide,BAC,Bis(2-acrylamidoethyl) disulfide
- Synonyms
- 2,2′-(Bisacrylamido)diethyl disulfideBACBis(2-acrylamidoethyl) disulfide
Reversible Gel Cross-linking
N,N′-Bis(acryloyl)cystamine serves as an effective reversible cross-linker for polyacrylamide gels. This property is crucial for applications requiring the subsequent retrieval of separated biomolecules from the gel matrix.
Macromolecule Isolation
The disulfide bond within N,N′-Bis(acryloyl)cystamine can be cleaved using reducing agents such as dithiothreitol (DTT). This enables the gentle dissolution of the gel and the subsequent isolation of macromolecules like proteins or nucleic acids.
SDS-PAGE and Proteomics
As a cross-linking agent for polyacrylamide gels, it is particularly useful in techniques like SDS-PAGE, facilitating downstream proteomic analyses by simplifying sample recovery.
| Molecular weight | 260.38 |
|---|---|
| Empirical formula | C10H16N2O2S2 |
| Assay | ≥97.0% (HPLC) |
| Storage temperature | 2-8°C |
| Application | BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. |
| Suitability | in accordance for electrophoresis test |
| Melting point | 121-125 °C |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
| Cation traces | Ca: ≤1000 mg/kgCd: ≤5 mg/kgCo: ≤5 mg/kgCr: ≤5 mg/kgCu: ≤5 mg/kgFe: ≤5 mg/kgK: ≤50 mg/kgMg: ≤50 mg/kgMn: ≤5 mg/kgNa: ≤2000 mg/kgNi: ≤5 mg/kgPb: ≤5 mg/kgZn: ≤5 mg/kg |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N,N′-Bis(acryloyl)cystamine used for?
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N,N′-Bis(acryloyl)cystamine is used as a reversible cross-linker for polyacrylamide gels, aiding in the isolation of macromolecules separated by electrophoresis.
What are the CAS number and formula for N,N′-Bis(acryloyl)cystamine?
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The CAS number is 60984-57-8, and its empirical formula (Hill notation) is C10H16N2O2S2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies N,N′-Bis(acryloyl)cystamine with an assay of ≥97.0% (HPLC). This product is designated as BioReagent grade and is not represented as USP, BP, EP, or other pharmacopoeia grades.
How should N,N′-Bis(acryloyl)cystamine be handled safely?
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N,N′-Bis(acryloyl)cystamine is classified with GHS07, carries the signal word Warning, and has hazard statements H315, H319, and H335, indicating it causes skin irritation, serious eye irritation, and may cause respiratory irritation. Recommended personal protective equipment includes a dust mask (N95), eyeshields, and gloves. Ensure adequate ventilation.
Is N,N′-Bis(acryloyl)cystamine available for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including N,N′-Bis(acryloyl)cystamine, and can fulfil export orders.
How can I request a quote or sample?
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Please contact our sales team directly through our website or by phone to request a quote or a sample of N,N′-Bis(acryloyl)cystamine.
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